A calcium- and light-gated switch to induce gene expression in activated neurons
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Dongmin | - |
dc.contributor.author | Hyun, Jung Ho | - |
dc.contributor.author | Jung, Kanghoon | - |
dc.contributor.author | Hannan, Patrick | - |
dc.contributor.author | Kwon, Hyung-Bae | - |
dc.date.accessioned | 2021-09-03T02:32:32Z | - |
dc.date.available | 2021-09-03T02:32:32Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-09 | - |
dc.identifier.issn | 1087-0156 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82437 | - |
dc.description.abstract | Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. We present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and brain slices, we show that Cal-Light drives expression of the reporter EGFP with high spatiotemporal resolution only in the presence of both blue light and calcium. Delivery of the Cal-Light components to the motor cortex of mice by viral vectors labels a subset of excitatory and inhibitory neurons related to learned lever-pressing behavior. By using Cal-Light to drive expression of the inhibitory receptor halorhodopsin (eNpHR), which responds to yellow light, we temporarily inhibit the lever-pressing behavior, confirming that the labeled neurons mediate the behavior. Thus, Cal-Light enables dissection of neural circuits underlying complex mammalian behaviors with high spatiotemporal precision. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | NERVOUS-SYSTEM | - |
dc.subject | CIRCUITS | - |
dc.subject | PROTEINS | - |
dc.subject | CULTURES | - |
dc.title | A calcium- and light-gated switch to induce gene expression in activated neurons | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Dongmin | - |
dc.identifier.doi | 10.1038/nbt.3902 | - |
dc.identifier.scopusid | 2-s2.0-85029308129 | - |
dc.identifier.wosid | 000410371600020 | - |
dc.identifier.bibliographicCitation | NATURE BIOTECHNOLOGY, v.35, no.9, pp.858 - 863 | - |
dc.relation.isPartOf | NATURE BIOTECHNOLOGY | - |
dc.citation.title | NATURE BIOTECHNOLOGY | - |
dc.citation.volume | 35 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 858 | - |
dc.citation.endPage | 863 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | CIRCUITS | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CULTURES | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.