Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Soojeong | - |
dc.contributor.author | Han, Daeyoung | - |
dc.contributor.author | Park, Min Chul | - |
dc.contributor.author | Mun, JiYoung | - |
dc.contributor.author | Choi, Jonghoon | - |
dc.contributor.author | Chun, Honggu | - |
dc.contributor.author | Kim, Sunghoon | - |
dc.contributor.author | Hong, Jong Wook | - |
dc.date.accessioned | 2021-09-03T02:49:45Z | - |
dc.date.available | 2021-09-03T02:49:45Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-08-30 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82514 | - |
dc.description.abstract | Extracellular vesicles (EVs) are the cell-secreted nano-and micro-sized particles consisted of lipid bilayer containing nucleic acids and proteins for diagnosis and therapeutic applications. The inherent complexity of EVs is a source of heterogeneity in various potential applications of the biological nanovesicles including analysis. To diminish heterogeneity, EV should be isolated and separated according to their sizes and cargos. However, current technologies do not meet the requirements. We showed noninvasive and precise separation of EVs based on their sizes without any recognizable damages. We separated atto-liter volumes of biological nanoparticles through operation of the present system showing relatively large volume of sample treatment to milliliters within an hour. We observed distinct size and morphological differences of 30 to 100 nm of exosomes and apoptotic bodies through TEM analysis. Indeed, we confirmed the biological moiety variations through immunoblotting with noninvasively separated EVs opening new windows in study and application of the biological nanoparticles. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | FIELD-FLOW FRACTIONATION | - |
dc.subject | EXOSOMES | - |
dc.subject | VESICLES | - |
dc.subject | MICROVESICLES | - |
dc.subject | SIZE | - |
dc.subject | QUANTIFICATION | - |
dc.subject | DEVICE | - |
dc.title | Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chun, Honggu | - |
dc.identifier.doi | 10.1038/s41598-017-08826-w | - |
dc.identifier.scopusid | 2-s2.0-85028593016 | - |
dc.identifier.wosid | 000408622400001 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.7 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | FIELD-FLOW FRACTIONATION | - |
dc.subject.keywordPlus | EXOSOMES | - |
dc.subject.keywordPlus | VESICLES | - |
dc.subject.keywordPlus | MICROVESICLES | - |
dc.subject.keywordPlus | SIZE | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | DEVICE | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.