Crystal structure of tRNA(His) guanylyltransferase from Saccharomyces cerevisiae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Kitaik | - |
dc.contributor.author | Lee, Eun Hye | - |
dc.contributor.author | Son, Jonghyeon | - |
dc.contributor.author | Hwang, Kwang Yeon | - |
dc.date.accessioned | 2021-09-03T02:54:21Z | - |
dc.date.available | 2021-09-03T02:54:21Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-08-19 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82540 | - |
dc.description.abstract | tRNA maturation involves several steps, including processing, splicing, CCA addition, and post transcriptional modifications. tRNA(His) guanylyltransferase (Thgl) is the only enzyme known to catalyze templated nucleotide addition in the 3'-5' direction, unlike other DNA and RNA polymerases. For a better understanding of its unique catalytic mechanism at the molecular level, we determined the crystal structure of GTP-bound Thgl from Saccharomyces cerevisiae at the maximum resolution of 3.0 angstrom. The structure revealed the enzyme to have a tetrameric conformation that is well conserved among different species, and the GTP molecule was clearly bound at the active site, coordinating with two magnesium ions. In addition, two flexible protomers at the potential binding site (PBS) for tRNA(His) were observed. We suggest that the PBS of the tetramer could also be one of the sites for interaction with partner proteins. (C) 2017 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | TRANSFER-RNA | - |
dc.subject | NUCLEOTIDE ADDITION | - |
dc.subject | POLYMERIZATION | - |
dc.subject | RECOGNITION | - |
dc.subject | CATALYZES | - |
dc.subject | THG1 | - |
dc.title | Crystal structure of tRNA(His) guanylyltransferase from Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Kwang Yeon | - |
dc.identifier.doi | 10.1016/j.bbrc.2017.06.054 | - |
dc.identifier.scopusid | 2-s2.0-85020769669 | - |
dc.identifier.wosid | 000406646600049 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.490, no.2, pp.400 - 405 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 490 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 400 | - |
dc.citation.endPage | 405 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | TRANSFER-RNA | - |
dc.subject.keywordPlus | NUCLEOTIDE ADDITION | - |
dc.subject.keywordPlus | POLYMERIZATION | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | CATALYZES | - |
dc.subject.keywordPlus | THG1 | - |
dc.subject.keywordAuthor | Thgl | - |
dc.subject.keywordAuthor | Reverse polymerization | - |
dc.subject.keywordAuthor | Posttranscriptional modifications | - |
dc.subject.keywordAuthor | GTP | - |
dc.subject.keywordAuthor | X-ray structure | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.