Inhibition of ubiquitin-specific protease 34 (USP34) induces epithelial-mesenchymal transition and promotes sternness in mammary epithelial cells
DC Field | Value | Language |
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dc.contributor.author | Oh, Eunhye | - |
dc.contributor.author | Kim, Ji Young | - |
dc.contributor.author | Sung, Daeil | - |
dc.contributor.author | Cho, Youngkwan | - |
dc.contributor.author | Lee, Nahyun | - |
dc.contributor.author | An, Hyunsook | - |
dc.contributor.author | Kim, Yoon-Jae | - |
dc.contributor.author | Cho, Tae-Min | - |
dc.contributor.author | Seo, Jae Hong | - |
dc.date.accessioned | 2021-09-03T03:16:48Z | - |
dc.date.available | 2021-09-03T03:16:48Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 0898-6568 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82624 | - |
dc.description.abstract | Ubiquitin-specific protease 34 (USP34) is a deubiquitinating enzyme that regulates Axin stability and plays a critical role in Wnt/beta-catenin signaling. We sought to investigate the role of USP34 on epithelial-mesenchymal (EMT) induction and its effects on mammary epithelial stem cells. USP34 expression levels were relatively lower in MDA-MB-231 and 4T1 mesenchymal-like cells when compared to epithelial-like cells. Inhibition of USP34 in NMuMG cells induced EMT, as evidenced by the upregulation of EMT markers including N-cadherin, phospho-Smad3, Snail and active-beta-catenin, as well as the downregulation of Axin 1 and E-cadherin. USP34 knockdown (KD) in these cells also resulted in the acquisition of invasive behavior, and promoted sternness as indicated by enhanced mammosphere-forming ability, concomitant with the upregulation of Nanog, Oct4 and Sox2 mRNA expression. Endogenous USP34 expression was observed to be at low levels in virgin mouse mammary glands in vivo. When USP34-KD cells were transplanted into the cleared mammary fat pads (CFP) of mice, these cells reconstituted the mammary gland with ductal tree development within 3 months. Our findings suggest a previously unknown role for USP34 in mammary gland development. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | STEM-CELLS | - |
dc.subject | BREAST-CANCER | - |
dc.subject | GLAND DEVELOPMENT | - |
dc.subject | BETA-CATENIN | - |
dc.subject | DEUBIQUITINATING ENZYMES | - |
dc.subject | TGF-BETA/BMP | - |
dc.subject | MORPHOGENESIS | - |
dc.subject | SYSTEM | - |
dc.subject | AXIN | - |
dc.subject | DIFFERENTIATION | - |
dc.title | Inhibition of ubiquitin-specific protease 34 (USP34) induces epithelial-mesenchymal transition and promotes sternness in mammary epithelial cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Ji Young | - |
dc.contributor.affiliatedAuthor | Kim, Yoon-Jae | - |
dc.contributor.affiliatedAuthor | Seo, Jae Hong | - |
dc.identifier.doi | 10.1016/j.cellsig.2017.05.009 | - |
dc.identifier.scopusid | 2-s2.0-85019246909 | - |
dc.identifier.wosid | 000405044000023 | - |
dc.identifier.bibliographicCitation | CELLULAR SIGNALLING, v.36, pp.230 - 239 | - |
dc.relation.isPartOf | CELLULAR SIGNALLING | - |
dc.citation.title | CELLULAR SIGNALLING | - |
dc.citation.volume | 36 | - |
dc.citation.startPage | 230 | - |
dc.citation.endPage | 239 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | GLAND DEVELOPMENT | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | DEUBIQUITINATING ENZYMES | - |
dc.subject.keywordPlus | TGF-BETA/BMP | - |
dc.subject.keywordPlus | MORPHOGENESIS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | AXIN | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordAuthor | USP34 | - |
dc.subject.keywordAuthor | EMT | - |
dc.subject.keywordAuthor | Sternness | - |
dc.subject.keywordAuthor | Mammary gland development | - |
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