MIR-27a regulates the TGF- signaling pathway by targeting SMAD2 and SMAD4 in lung cancer
- Authors
- Chae, Dong-Kyu; Ban, Eunmi; Yoo, Young Sook; Kim, Eunice EunKyeong; Baik, Ja-Hyun; Song, Eun Joo
- Issue Date
- 8월-2017
- Publisher
- WILEY
- Keywords
- lung cancer; miR-27a; SMAD2; SMAD4; TGF-
- Citation
- MOLECULAR CARCINOGENESIS, v.56, no.8, pp.1992 - 1998
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR CARCINOGENESIS
- Volume
- 56
- Number
- 8
- Start Page
- 1992
- End Page
- 1998
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/82650
- DOI
- 10.1002/mc.22655
- ISSN
- 0899-1987
- Abstract
- The transforming growth factor- (TGF-) signaling pathway is associated with carcinogenesis and various biological processes. SMAD2 and SMAD4, which are putative tumor suppressors, have an important role in TGF- signaling. The aberrant expression of these genes is implicated in some cancers. However, the mechanisms of SMAD2 and SMAD4 dysregulation are poorly understood. In this study, we observed that miR-27a was upregulated in lung cancer cell lines and patients. In addition, SMAD2 and SMAD4 genes were identified as targets of miR-27a by several target prediction databases and experimental validation. Functional studies revealed that miR-27a overexpression decreased SMAD2 and SMAD4 mRNA and protein levels. Furthermore, miR-27a contributed to cell proliferation and invasion by inhibiting TGF--induced cell cycle arrest. These results suggest that miR-27a may function as an oncogene by regulating SMAD2 and SMAD4 in lung cancer. Thus, miR-27a may be a potential target for cancer therapy.
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