Myofibroblast in the ligamentum flavum hypertrophic activity
DC Field | Value | Language |
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dc.contributor.author | Hur, Junseok W. | - |
dc.contributor.author | Bae, Taegeun | - |
dc.contributor.author | Ye, Sunghyeok | - |
dc.contributor.author | Kim, Joo-Hyun | - |
dc.contributor.author | Lee, Sunhye | - |
dc.contributor.author | Kim, Kyoungmi | - |
dc.contributor.author | Lee, Seung-Hwan | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Lee, Jang-Bo | - |
dc.contributor.author | Cho, Tai-Hyoung | - |
dc.contributor.author | Park, Jung-Yul | - |
dc.contributor.author | Hur, Junho K. | - |
dc.date.accessioned | 2021-09-03T03:42:10Z | - |
dc.date.available | 2021-09-03T03:42:10Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 0940-6719 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82768 | - |
dc.description.abstract | Majority of the previous studies compared lumbar spinal stenosis (LSS) and lumbar disc herniation (LDH) patients for analyses of LFH. However, the separation of normal/hypertrophied LF has often been ambiguous and the severity of hypertrophic activity differed. Here, we present a novel analysis scheme for LFH in which myofibroblast is proposed as a major etiological factor for LFH study. Seventy-one LF patient tissue samples were used for this study. Initially, mRNA levels of the samples were assessed by qRT-PCR: angiopoietin-like protein-2 (ANGPTL2), transforming growth factor-beta1 (TGF-beta 1), vascular endothelial growth factor (VEGF), interleukin-6, collagen-1, 3, 4, 5, and 11, and elastin. Myofibroblasts were detected by immune stain using alpha-smooth muscle actin (alpha SMA) as a marker. To study the myofibroblast in TGF-beta pathway, LF tissues were analyzed for protein levels of alpha SMA/TGF-beta 1 by Western blot. In addition, from LF cells cultured with exogenous TGF-beta 1 conditioned medium, expression of alpha SMA/collagen-1 was assessed and the cell morphology was identified. The comparative analysis of mRNA expression levels (LSS vs LDH) failed to show significant differences in TGF-beta 1 (p = 0.08); however, we found a significant positive correlation among ANGPTL2, VEGF, TGF-beta 1, and collagen-1 and 3, which represent common trends in hypertrophic activity (p < 0.05). We detected myofibroblast in the patient samples by alpha SMA staining, and the protein levels of alpha SMA were positively correlated with TGF-beta 1. In LF cell culture, exogenous TGF-beta 1 upregulated alpha SMA/collagen-1 mRNA levels and facilitated trans-differentiation to myofibroblast. We conclude that the transition of fibroblast to myofibroblasts via TGF-beta pathway is a key linker between inflammation and fibrosis in LFH mechanism. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | SPINAL-CANAL STENOSIS | - |
dc.subject | TISSUE | - |
dc.subject | PATHOGENESIS | - |
dc.subject | DISEASES | - |
dc.subject | FIBROSIS | - |
dc.title | Myofibroblast in the ligamentum flavum hypertrophic activity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hur, Junseok W. | - |
dc.contributor.affiliatedAuthor | Kim, Kyoungmi | - |
dc.contributor.affiliatedAuthor | Lee, Jang-Bo | - |
dc.contributor.affiliatedAuthor | Cho, Tai-Hyoung | - |
dc.contributor.affiliatedAuthor | Park, Jung-Yul | - |
dc.identifier.doi | 10.1007/s00586-017-4981-2 | - |
dc.identifier.scopusid | 2-s2.0-85011856655 | - |
dc.identifier.wosid | 000407369400005 | - |
dc.identifier.bibliographicCitation | EUROPEAN SPINE JOURNAL, v.26, no.8, pp.2021 - 2030 | - |
dc.relation.isPartOf | EUROPEAN SPINE JOURNAL | - |
dc.citation.title | EUROPEAN SPINE JOURNAL | - |
dc.citation.volume | 26 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2021 | - |
dc.citation.endPage | 2030 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalResearchArea | Orthopedics | - |
dc.relation.journalWebOfScienceCategory | Clinical Neurology | - |
dc.relation.journalWebOfScienceCategory | Orthopedics | - |
dc.subject.keywordPlus | SPINAL-CANAL STENOSIS | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordPlus | FIBROSIS | - |
dc.subject.keywordAuthor | Ligamentum flavum | - |
dc.subject.keywordAuthor | Hypertrophy | - |
dc.subject.keywordAuthor | Myofibroblasts | - |
dc.subject.keywordAuthor | Alpha-smooth muscle actin | - |
dc.subject.keywordAuthor | Transforming growth factor beta1 | - |
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