Identification of candidate genes for the seed coat colour change in a Brachypodium distachyon mutant induced by gamma radiation using whole-genome re-sequencing
- Authors
- Lee, Man Bo; Kim, Dae Yeon; Seo, Yong Weon
- Issue Date
- 7월-2017
- Publisher
- CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
- Keywords
- Brachypodium distachyon; gamma radiation; SNP; InDel; seed coat colour
- Citation
- GENOME, v.60, no.7, pp.581 - 587
- Indexed
- SCIE
SCOPUS
- Journal Title
- GENOME
- Volume
- 60
- Number
- 7
- Start Page
- 581
- End Page
- 587
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/82915
- DOI
- 10.1139/gen-2016-0145
- ISSN
- 0831-2796
- Abstract
- Brachypodium distachyon has been proposed as a model plant for agriculturally important cereal crops such as wheat and barley. Seed coat colour change from brown-red to yellow was observed in a mutant line (142-3) of B. distachyon, which was induced by chronic gamma radiation. In addition, dwarf phenotypes were observed in each of the lines 142-3, 421-2, and 1376-1. To identify causal mutations for the seed coat colour change, the three mutant lines and the wild type were subjected to whole-genome re-sequencing. After removing natural variations, 906, 1057, and 978 DNA polymorphisms were detected in 142-3, 421-2, and 1376-1, respectively. A total of 13 high-risk DNA polymorphisms were identified in mutant 142-3. Based on a comparison with DNA polymorphisms in 421-2 and 1376-1, candidate causal mutations for the seed coat colour change in 142-3 were selected. In the two independent Arabidopsis thaliana lines carrying T-DNA insertions in the AtCHI, seed colour change was observed. We propose a frameshift mutation in BdCHI1 as a causal mutation responsible for seed colour change in 142-3. The DNA polymorphism information for these mutant lines can be utilized for functional genomics in B. distachyon and cereal crops.
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Collections - Graduate School > Department of Plant Biotechnology > 1. Journal Articles
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