Grape seed proanthocyanidin inhibits inflammatory responses in hepatic stellate cells by modulating the MAPK, Akt and NF-kappa B signaling pathways
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jin-Woo | - |
dc.contributor.author | Kim, Young Il | - |
dc.contributor.author | Kim, Youngchul | - |
dc.contributor.author | Choi, Minji | - |
dc.contributor.author | Min, Seoyeon | - |
dc.contributor.author | Joo, Yong Hoon | - |
dc.contributor.author | Yim, Sung-Vin | - |
dc.contributor.author | Chung, Namhyun | - |
dc.date.accessioned | 2021-09-03T04:24:01Z | - |
dc.date.available | 2021-09-03T04:24:01Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-07 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82934 | - |
dc.description.abstract | In the present study, we aimed to investigate the molecular mechanisms and prophylactic effects of grape seed proanthocyanidin (GSP) on lipopolysaccharide (LPS)-stimulated human hepatic stellate cells (HSCs). Cell counting and MTT assays were used to assess cell viability in the absence or presence of GSP. Reverse transcription-quantitative PCR (RT-qPCR) was performed for several inflammation-related genes (NOD1, NOD2, TLR2, TLR4, IL-1 , IL-6, IL-8, iNOS and COX-2). The expression of anti-inflammatory cell signaling molecules, including c-Jun N-terminal kinase (JNK), p38, extracellular signal regulated kinase (ERK), Akt, nuclear factor-B (NF-B), inhibitory-B (IB), iNOS and COX-2, was evaluated by western blot analysis. Finally, IL-8 levels in the culture supernatant of HSCs were measured by ELISA. Pretreatment with GSP before LPS treatment significantly suppressed the mRNA expression of pro-inflammatory cytokines such as IL-1, IL-6 and IL-8. GSP inhibited mRNA expression of LPS-induced TLR4, NOD2 and COX-2, in addition to inhibiting the expression of iNOS. GSP also inhibited LPS-induced NF-B activation and IB phosphorylation. Concomitantly, GSP dose-dependently suppressed the activation of MAP kinases (JNK, ERK and p38) and Akt in LPS-stimulated HSCs. These data suggest that GSP inhibits inflammatory responses in HSCs by inactivating the NF-B signaling pathway via MAP kinases. Thus, GSP may be considered as a novel drug for the treatment of hepatic inflammation, infectious diseases and fibrosis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.subject | LIVER FIBROSIS | - |
dc.subject | EXTRACT | - |
dc.subject | EXPRESSION | - |
dc.subject | ACTIVATION | - |
dc.subject | CHEMOATTRACTANT | - |
dc.subject | MACROPHAGES | - |
dc.subject | MECHANISMS | - |
dc.subject | RESOLUTION | - |
dc.subject | RECEPTORS | - |
dc.subject | DISEASE | - |
dc.title | Grape seed proanthocyanidin inhibits inflammatory responses in hepatic stellate cells by modulating the MAPK, Akt and NF-kappa B signaling pathways | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Namhyun | - |
dc.identifier.doi | 10.3892/ijmm.2017.2997 | - |
dc.identifier.scopusid | 2-s2.0-85020789526 | - |
dc.identifier.wosid | 000404886700026 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.40, no.1, pp.226 - 234 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.volume | 40 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 226 | - |
dc.citation.endPage | 234 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | LIVER FIBROSIS | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CHEMOATTRACTANT | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordAuthor | cyclooxygenase-2 | - |
dc.subject.keywordAuthor | grape seed proanthocyanidin | - |
dc.subject.keywordAuthor | hepatic stellate cells | - |
dc.subject.keywordAuthor | inducible nitric oxide synthase | - |
dc.subject.keywordAuthor | mitogen-activated protein kinase | - |
dc.subject.keywordAuthor | Toll-like receptors | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.