WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyung Chul | - |
dc.contributor.author | Jung, Seung Hee | - |
dc.contributor.author | Hwang, Hyun Jung | - |
dc.contributor.author | Kang, Donghee | - |
dc.contributor.author | De, Supriyo | - |
dc.contributor.author | Dudekula, Dawood B. | - |
dc.contributor.author | Martindale, Jennifer L. | - |
dc.contributor.author | Park, Byungkyu | - |
dc.contributor.author | Park, Seung Kuk | - |
dc.contributor.author | Lee, Eun Kyung | - |
dc.contributor.author | Lee, Jeong-Hwa | - |
dc.contributor.author | Jeong, Sunjoo | - |
dc.contributor.author | Han, Kyungsook | - |
dc.contributor.author | Park, Heon Joo | - |
dc.contributor.author | Ko, Young-Gyu | - |
dc.contributor.author | Gorospe, Myriam | - |
dc.contributor.author | Lee, Jae-Seon | - |
dc.date.accessioned | 2021-09-03T04:54:48Z | - |
dc.date.available | 2021-09-03T04:54:48Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-06-20 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83106 | - |
dc.description.abstract | RNA-binding proteins (RBPs) are involved in mRNA splicing, maturation, transport, translation, storage and turnover. Here, we identified ACOT7 mRNA as a novel target of human WIG1. ACOT7 mRNA decay was triggered by the microRNA miR-9 in a WIG1-dependent manner via classic recruitment of Argonaute 2 (AGO2). Interestingly, AGO2 was also recruited to ACOT7 mRNA in a WIG1-dependent manner in the absence of miR-9, which indicates an alternative model whereby WIG1 controls AGO2-mediated gene silencing. The WIG1-AGO2 complex attenuated translation initiation via an interaction with translation initiation factor 5B (eIF5B). These results were confirmed using a WIG1 tethering system based on the MS2 bacteriophage coat protein and a reporter construct containing an MS2-binding site, and by immunoprecipitation of WIG1 and detection of WIG1-associated proteins using liquid chromatography-tandem mass spectrometry. We also identified WIG1-binding motifs using photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation analyses. Altogether, our data indicate that WIG1 governs the miRNA-dependent and the miRNA-independent recruitment of AGO2 to lower the stability of and suppress the translation of ACOT7 mRNA. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | P53 TARGET WIG-1 | - |
dc.subject | BINDING PROTEINS | - |
dc.subject | TRANSLATIONAL REPRESSION | - |
dc.subject | WIDE IDENTIFICATION | - |
dc.subject | MICRORNA | - |
dc.subject | GENE | - |
dc.subject | ARGONAUTE | - |
dc.subject | REQUIRES | - |
dc.subject | GW182 | - |
dc.subject | DICER | - |
dc.title | WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ko, Young-Gyu | - |
dc.identifier.doi | 10.1093/nar/gkx307 | - |
dc.identifier.scopusid | 2-s2.0-85027148732 | - |
dc.identifier.wosid | 000403693000062 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.45, no.11, pp.6894 - 6910 | - |
dc.relation.isPartOf | NUCLEIC ACIDS RESEARCH | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 45 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 6894 | - |
dc.citation.endPage | 6910 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | P53 TARGET WIG-1 | - |
dc.subject.keywordPlus | BINDING PROTEINS | - |
dc.subject.keywordPlus | TRANSLATIONAL REPRESSION | - |
dc.subject.keywordPlus | WIDE IDENTIFICATION | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | ARGONAUTE | - |
dc.subject.keywordPlus | REQUIRES | - |
dc.subject.keywordPlus | GW182 | - |
dc.subject.keywordPlus | DICER | - |
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