Metabolic Design of Corynebacterium glutamicum for Production of L-Cysteine with Consideration of Sulfur-Supplemented Animal Feed
- Authors
- Joo, Young-Chul; Hyeon, Jeong Eun; Han, Sung Ok
- Issue Date
- 14-6월-2017
- Publisher
- AMER CHEMICAL SOC
- Keywords
- sulfur-containing amino acid; animal feed supplements; L-cysteine production; metabolic engineering; Corynebacterium glutamicum
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.65, no.23, pp.4698 - 4707
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Volume
- 65
- Number
- 23
- Start Page
- 4698
- End Page
- 4707
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/83134
- DOI
- 10.1021/acs.jafc.7b01061
- ISSN
- 0021-8561
- Abstract
- L-Cysteine is a valuable sulfur-containing amino acid widely used as a nutrition supplement in industrial food production, agriculture, and animal feed. However, this amino acid is mostly produced by acid hydrolysis and extraction from human or animal hairs. In this study, we constructed recombinant Corynebacterium glutamicum strains that overexpress combinatorial genes for L-cysteine production. The aims of this work were to investigate the effect of the combined overexpression of serine acetyltransferase (CysE), O-acetylserine sulfhydrylase (CysK), and the transcriptional regulator CysR on L-cysteine production. The CysR-overexpressing strain accumulated approximately 2.7-fold more intracellular sulfide than the control strain (empty pMT-tac vector). Moreover-, in the resulting CysEKR recombinant strain, combinatorial overexpression of genes involved in L-cysteine production successfully enhanced its production by approximately 3.0-fold relative to that in the control strain. This study demonstrates a biotechnological model for the production of animal feed supplements such as Lcysteine using metabolically engineered C. glutamicum.
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Collections - College of Life Sciences and Biotechnology > Division of Biotechnology > 1. Journal Articles
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