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Pure enzyme cocktails tailored for the saccharification of sugarcane bagasse pretreated by using different methods

Authors
Kim, In JungLee, Hee JinKim, Kyoung Heon
Issue Date
6월-2017
Publisher
ELSEVIER SCI LTD
Keywords
Cellulase cocktail; Pretreatment; Lignocellulose; Sugarcane bagasse; Mixture design
Citation
PROCESS BIOCHEMISTRY, v.57, pp.167 - 174
Indexed
SCIE
SCOPUS
Journal Title
PROCESS BIOCHEMISTRY
Volume
57
Start Page
167
End Page
174
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/83214
DOI
10.1016/j.procbio.2017.04.006
ISSN
1359-5113
Abstract
The compositions and physical properties of pretreated lignocellulose vary depending on pretreatment methods; therefore, enzyme cocktails specific to pretreatments are desired for efficient saccharification of lignocellulose. Here, enzyme cocktails consisting of three pure lignocellulolytic enzymes endoglucanase (EG), cellobiohydrolase (CBH) and endoxylanase (XN) with a fixed amount of beta-glucosidase were tailored for acid- and alkali-pretreated sugarcane bagasse (ACID and ALKALI, respectively). Based on a mixture design, the optimal mass ratios of EG, CBH, and XN were determined to be 61.25:38.73:0.02 and 53.99:34.60:11.41 for ACID and ALKALI, respectively. The optimized enzyme cocktail yielded a higher or comparable amount of reducing sugars from the hydrolysis of ACID and ALKALI when compared to that obtained using commercial cellulase mixtures. Using the commercial and easily available pure enzymes, this simple method for the in-house preparation of an enzyme cocktail specific to pretreated lignocellulose consisting of only four enzymes with a high level of hydrolysis will be helpful for achieving enzymatic saccharification in the lignocellulose-based biorefinery.
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