Immunologic characteristics of human gingival fibroblasts in response to oral bacteria
DC Field | Value | Language |
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dc.contributor.author | Jang, J. Y. | - |
dc.contributor.author | Song, I. -S. | - |
dc.contributor.author | Baek, K. J. | - |
dc.contributor.author | Choi, Y. | - |
dc.contributor.author | Ji, S. | - |
dc.date.accessioned | 2021-09-03T05:35:06Z | - |
dc.date.available | 2021-09-03T05:35:06Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-06 | - |
dc.identifier.issn | 0022-3484 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83282 | - |
dc.description.abstract | Background and ObjectiveThere is ample evidence that gingival fibroblasts (GFs) participate in the immune response to oral bacteria and serve as immune-regulatory cells. The objective of this study was to investigate the innate immune response of GFs to oral bacteria. Material and MethodsHuman GFs were cocultured with relatively less-pathogenic (Leptotrichia wadei, Fusobacterium nucleatum and Campylobacter gracilis) and pathogenic red-complex bacteria. The expression of mRNA for antimicrobial peptides [AMPs; namely human beta defensins (HBDs)], chemokines with antimicrobial activity [chemokine C-X-C motif (CXCL)10, CXCL11 and chemokine C-C motif ligand 20 (CCL20)] and proinflammatory mediators [interleukin (IL)6 and IL8] and the levels of CXCL11, CCL20, IL-6 and IL-8 accumulated in supernatants were analyzed using real-time PCR and ELISA, respectively. The proteolytic activities of CXCL11, CCL20, IL-6 and IL-8 produced by six species of bacteria were also determined. ResultsThe relatively less-pathogenic bacteria strongly up-regulated the expression of antimicrobial chemokines and proinflammatory mediators, whereas the red-complex bacteria stimulated low levels, or often suppressed, expression of these factors. Regarding the regulation of AMPs, the inhibition of HBD3, HBD106 and HBD107 mRNAs by Porphyromonas gingivalis was noticeable; however, differences between the two bacterial groups were not conspicuous. Differential degradation of proteins by the six bacterial species was observed: P. gingivalis and Treponema denticola degraded proteins well, whereas the other species degraded proteins to a relatively lower degree. ConclusionThe invasion of red-complex bacteria into gingival connective tissue can suppress the immune response of GFs and can be a source of persistent infection in connective tissue. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | HUMAN BETA-DEFENSINS | - |
dc.subject | PORPHYROMONAS-GINGIVALIS | - |
dc.subject | PERIODONTAL-DISEASE | - |
dc.subject | ANTIMICROBIAL PEPTIDES | - |
dc.subject | CHEMOKINE RECEPTORS | - |
dc.subject | EPITHELIAL-CELLS | - |
dc.subject | TREPONEMA-DENTICOLA | - |
dc.subject | EXPRESSION | - |
dc.subject | HEALTH | - |
dc.subject | INNATE | - |
dc.title | Immunologic characteristics of human gingival fibroblasts in response to oral bacteria | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, I. -S. | - |
dc.identifier.doi | 10.1111/jre.12410 | - |
dc.identifier.scopusid | 2-s2.0-84983266557 | - |
dc.identifier.wosid | 000399955500016 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PERIODONTAL RESEARCH, v.52, no.3, pp.447 - 457 | - |
dc.relation.isPartOf | JOURNAL OF PERIODONTAL RESEARCH | - |
dc.citation.title | JOURNAL OF PERIODONTAL RESEARCH | - |
dc.citation.volume | 52 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 447 | - |
dc.citation.endPage | 457 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Dentistry, Oral Surgery & Medicine | - |
dc.relation.journalWebOfScienceCategory | Dentistry, Oral Surgery & Medicine | - |
dc.subject.keywordPlus | HUMAN BETA-DEFENSINS | - |
dc.subject.keywordPlus | PORPHYROMONAS-GINGIVALIS | - |
dc.subject.keywordPlus | PERIODONTAL-DISEASE | - |
dc.subject.keywordPlus | ANTIMICROBIAL PEPTIDES | - |
dc.subject.keywordPlus | CHEMOKINE RECEPTORS | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | TREPONEMA-DENTICOLA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | HEALTH | - |
dc.subject.keywordPlus | INNATE | - |
dc.subject.keywordAuthor | antimicrobial chemokines | - |
dc.subject.keywordAuthor | human gingival fibroblasts | - |
dc.subject.keywordAuthor | oral bacteria | - |
dc.subject.keywordAuthor | proinflammatory mediator | - |
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