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Detection of organophosphate bound butyrylcholinesterase using a monoclonal antibody

Authors
Park, Sang-JinKim, Dong-HwanYoo, JeonghaHwang, Eun YoungShin, Moon-SikNam-Taek LeeCho, Il-RaeKang, Hee-GunKim, Young-JinPark, SungmanKim, Yoon-Won
Issue Date
6월-2017
Publisher
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
Keywords
Butyrylcholinesterase; Diagnostic monoclonal antibody; Nerve toxic agents; Organophosphate
Citation
APPLIED BIOLOGICAL CHEMISTRY, v.60, no.3, pp.233 - 240
Indexed
SCIE
SCOPUS
KCI
Journal Title
APPLIED BIOLOGICAL CHEMISTRY
Volume
60
Number
3
Start Page
233
End Page
240
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/83364
DOI
10.1007/s13765-017-0272-1
ISSN
2468-0834
Abstract
In this study, a specific antibody against butyrylcholinesterase (BuChE) bound to organophosphate (OP) nerve agents was developed to be used in enzymelinked immunosorbent assays (ELISA). OP nerve agents such as sarin, soman, and VX are known to act on the neuromuscular junctions and were synthesized at the Armed Force Chemical, Biological, and Radiological Defense Command in Republic of Korea and studied after confirming inactivation of BuChE. Each inactivated OP-BuChE (OPiBuChE) was used as the antigen for developing monoclonal antibodies (mAbs) in mice for detection of OP bound BuChE. The fusion was performed after immunization and then hybridoma cells for the antibodies were generated. As a result, the partial specific antibody against soman-iBuChE was confirmed via ELISA. Thus, the antibody may recognize three-dimensional structure of OP-iBuChE that is changed upon soman and BuChE reaction, not an OP binding pocket of BuChE. Furthermore, this partial specific monoclonal antibody may be used in a competitive ELISA to detect the soman-iBuChE. Taken together, we suggest that the antibody could be applied to evaluate the soman presence in blood, serum, and urine.
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