Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR
DC Field | Value | Language |
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dc.contributor.author | Noh, Ji Yeong | - |
dc.contributor.author | Yoon, Sun-Woo | - |
dc.contributor.author | Kim, Doo-Jin | - |
dc.contributor.author | Lee, Moo-Seung | - |
dc.contributor.author | Kim, Ji-Hyung | - |
dc.contributor.author | Na, Woonsung | - |
dc.contributor.author | Song, Daesub | - |
dc.contributor.author | Jeong, Dae Gwin | - |
dc.contributor.author | Kim, Hye Kwon | - |
dc.date.accessioned | 2021-09-03T05:52:59Z | - |
dc.date.available | 2021-09-03T05:52:59Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-06 | - |
dc.identifier.issn | 0304-8608 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83384 | - |
dc.description.abstract | Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50-100 copies/mL and 5-100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER WIEN | - |
dc.subject | SARS | - |
dc.title | Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Daesub | - |
dc.identifier.doi | 10.1007/s00705-017-3281-9 | - |
dc.identifier.scopusid | 2-s2.0-85013168169 | - |
dc.identifier.wosid | 000401119300016 | - |
dc.identifier.bibliographicCitation | ARCHIVES OF VIROLOGY, v.162, no.6, pp.1617 - 1623 | - |
dc.relation.isPartOf | ARCHIVES OF VIROLOGY | - |
dc.citation.title | ARCHIVES OF VIROLOGY | - |
dc.citation.volume | 162 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1617 | - |
dc.citation.endPage | 1623 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordPlus | SARS | - |
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