Identification of amino acids within norovirus polymerase involved in RNA binding and viral replication
DC Field | Value | Language |
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dc.contributor.author | Han, Kang Rok | - |
dc.contributor.author | Alhatlani, Bader Y. | - |
dc.contributor.author | Cho, Sunyoung | - |
dc.contributor.author | Lee, Ji-Hye | - |
dc.contributor.author | Hosmillo, Myra | - |
dc.contributor.author | Goodfellow, Ian G. | - |
dc.contributor.author | Kim, Kyung Hyun | - |
dc.contributor.author | Yang, Jai Myung | - |
dc.date.accessioned | 2021-09-03T05:57:15Z | - |
dc.date.available | 2021-09-03T05:57:15Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-06 | - |
dc.identifier.issn | 0022-1317 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83409 | - |
dc.description.abstract | Until recently, molecular studies on human norovirus (HuNoV), a major causative agent of gastroenteritis, have been hampered by the lack of an efficient cell culture system. Murine norovirus-1 (MNV-1) has served as a surrogate model system for norovirus research, due to the availability of robust cell culture systems and reverse genetics. To identify amino acids involved in RNA synthesis by the viral RNA-dependent RNA polymerase (NS7), we constructed NS7 mutants in which basic amino acids surrounding the catalytic site were substituted with alanine. Electrophoretic mobility shift assay revealed that these residues are important for RNA binding, particularly R-396. Furthermore, in vitro RNA synthesis and reverse genetics were used to identify conserved amino acids essential for RNA synthesis and viral replication. These results provide additional functional insights into highly conserved amino acids in NS7 and provide potential methods of rational attenuation of norovirus replication. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MICROBIOLOGY SOC | - |
dc.subject | HEPATITIS-C VIRUS | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | MURINE NOROVIRUS | - |
dc.subject | ACTIVE-SITE | - |
dc.subject | REVEALS | - |
dc.subject | PROTEIN | - |
dc.subject | CELLS | - |
dc.subject | VPG | - |
dc.subject | CALICIVIRUS | - |
dc.subject | INITIATION | - |
dc.title | Identification of amino acids within norovirus polymerase involved in RNA binding and viral replication | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hyun | - |
dc.identifier.doi | 10.1099/jgv.0.000826 | - |
dc.identifier.scopusid | 2-s2.0-85023631572 | - |
dc.identifier.wosid | 000410020900020 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GENERAL VIROLOGY, v.98, no.6, pp.1311 - 1315 | - |
dc.relation.isPartOf | JOURNAL OF GENERAL VIROLOGY | - |
dc.citation.title | JOURNAL OF GENERAL VIROLOGY | - |
dc.citation.volume | 98 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1311 | - |
dc.citation.endPage | 1315 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordPlus | HEPATITIS-C VIRUS | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | MURINE NOROVIRUS | - |
dc.subject.keywordPlus | ACTIVE-SITE | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | VPG | - |
dc.subject.keywordPlus | CALICIVIRUS | - |
dc.subject.keywordPlus | INITIATION | - |
dc.subject.keywordAuthor | norovirus | - |
dc.subject.keywordAuthor | RNA binding | - |
dc.subject.keywordAuthor | RNA replication | - |
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