In vitro Multi-Species Biofilms of Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa and Their Host Interaction during In vivo Colonization of an Otitis Media Rat Model
DC Field | Value | Language |
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dc.contributor.author | Yadav, Mukesh K. | - |
dc.contributor.author | Chae, Sung-Won | - |
dc.contributor.author | Go, Yoon Young | - |
dc.contributor.author | Im, Gi Jung | - |
dc.contributor.author | Song, Jae-Jun | - |
dc.date.accessioned | 2021-09-03T07:08:59Z | - |
dc.date.available | 2021-09-03T07:08:59Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-04-18 | - |
dc.identifier.issn | 2235-2988 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83745 | - |
dc.description.abstract | Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) are known to cause biofilm-related infections. MRSA and PA have been frequently isolated from chronically infected wounds, cystic fibrosis, chronic suppurative otitis media (CSOM), and from indwelling medical devices, and these bacteria co-exist; however, their interaction with each other or with the host is not well known. In this study, we investigated MRSA and PA multi-species biofilm communities in vitro and their interaction with the host during in vivo colonization using an OM rat-model. In-vitro biofilm formation and in-vivo colonization were studied using CV-microtiter plate assay and OM rat-model respectively. The biofilms were viewed under scanning electron microscope and bacteria were enumerated using cfu counts. The differential gene expressions of rat mucosa colonized with single or multi-species of MRSA or PA were studied using RNA-sequencing of total transcriptome. In multi-species in-vitro biofilms PA partially inhibited SA growth. However, no significant inhibition of MRSA was detected during in vivocolonization of multi species in rat bullae. A total of 1,797 genes were significantly (p < 0.05) differentially expressed in MRSA or PA or MRSA + PA colonized rat middle ear mucosa with respect to the control. The poly-microbial colonization of MRSA and PA induced the differential expression of a significant number of genes that are involved in immune response, inflammation, signaling, development, and defense; these were not expressed with single species colonization by either MRSA or PA. Genes involved in defense, immune response, inflammatory response, and developmental process were exclusively up-regulated, and genes that are involved in nervous system signaling, development and transmission, regulation of cell growth and development, anatomical and system development, and cell differentiation were down-regulated after multi-species inoculation. These results indicate that poly-microbial colonization induces a host response that is different from that induced by single species infection. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.subject | SMALL-COLONY VARIANTS | - |
dc.subject | CYSTIC-FIBROSIS SPUTUM | - |
dc.subject | CHRONIC RHINOSINUSITIS | - |
dc.subject | INFECTIONS | - |
dc.subject | GROWTH | - |
dc.subject | ALGINATE | - |
dc.subject | EXPRESSION | - |
dc.subject | CHILDREN | - |
dc.subject | DEVICES | - |
dc.subject | SURGERY | - |
dc.title | In vitro Multi-Species Biofilms of Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa and Their Host Interaction during In vivo Colonization of an Otitis Media Rat Model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Go, Yoon Young | - |
dc.contributor.affiliatedAuthor | Im, Gi Jung | - |
dc.contributor.affiliatedAuthor | Song, Jae-Jun | - |
dc.identifier.doi | 10.3389/fcimb.2017.00125 | - |
dc.identifier.scopusid | 2-s2.0-85027455752 | - |
dc.identifier.wosid | 000399428900001 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v.7 | - |
dc.relation.isPartOf | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | - |
dc.citation.title | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | - |
dc.citation.volume | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | SMALL-COLONY VARIANTS | - |
dc.subject.keywordPlus | CYSTIC-FIBROSIS SPUTUM | - |
dc.subject.keywordPlus | CHRONIC RHINOSINUSITIS | - |
dc.subject.keywordPlus | INFECTIONS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | ALGINATE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CHILDREN | - |
dc.subject.keywordPlus | DEVICES | - |
dc.subject.keywordPlus | SURGERY | - |
dc.subject.keywordAuthor | biofilms | - |
dc.subject.keywordAuthor | planktonic | - |
dc.subject.keywordAuthor | otitis media | - |
dc.subject.keywordAuthor | methicillin resistant Staphylococcus aureus | - |
dc.subject.keywordAuthor | Pseudomonas aeruginosa | - |
dc.subject.keywordAuthor | poly-microbial | - |
dc.subject.keywordAuthor | colonization | - |
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