Acetoin Production Using Metabolically Engineered Klebsiella pneumoniae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Ji-Woong | - |
dc.contributor.author | Jung, Hwi-Min | - |
dc.contributor.author | Kim, Duck Gyun | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-03T07:41:22Z | - |
dc.date.available | 2021-09-03T07:41:22Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 0304-128X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83924 | - |
dc.description.abstract | Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation. | - |
dc.language | Korean | - |
dc.language.iso | ko | - |
dc.publisher | KOREAN INSTITUTE CHEMICAL ENGINEERS | - |
dc.subject | SERRATIA-MARCESCENS H32 | - |
dc.subject | WABG GENE | - |
dc.subject | OPTIMIZATION | - |
dc.subject | VIRULENCE | - |
dc.subject | STRAIN | - |
dc.title | Acetoin Production Using Metabolically Engineered Klebsiella pneumoniae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.9713/kcer.2017.55.2.237 | - |
dc.identifier.scopusid | 2-s2.0-85016984664 | - |
dc.identifier.wosid | 000424599300013 | - |
dc.identifier.bibliographicCitation | KOREAN CHEMICAL ENGINEERING RESEARCH, v.55, no.2, pp.237 - 241 | - |
dc.relation.isPartOf | KOREAN CHEMICAL ENGINEERING RESEARCH | - |
dc.citation.title | KOREAN CHEMICAL ENGINEERING RESEARCH | - |
dc.citation.volume | 55 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 237 | - |
dc.citation.endPage | 241 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002207808 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | SERRATIA-MARCESCENS H32 | - |
dc.subject.keywordPlus | WABG GENE | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | VIRULENCE | - |
dc.subject.keywordPlus | STRAIN | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Acetoin | - |
dc.subject.keywordAuthor | 2,3-Butanediol dehydrogenase | - |
dc.subject.keywordAuthor | NADH oxidase | - |
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