Generation of a monoclonal antibody that has reduced binding activity to VX-inactivated butyrylcholinesterase (BuChE) compared to BuChE by phage display
DC Field | Value | Language |
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dc.contributor.author | Yoon, Jun-Yeol | - |
dc.contributor.author | Kim, Dong-Hwan | - |
dc.contributor.author | Kim, Sangkyu | - |
dc.contributor.author | Kim, Dain | - |
dc.contributor.author | Jo, Gyunghee | - |
dc.contributor.author | Shin, Moon-Sik | - |
dc.contributor.author | Yoo, Jeongha | - |
dc.contributor.author | Kang, Heui Keun | - |
dc.contributor.author | Kim, Min Soo | - |
dc.contributor.author | Kim, Young-Jin | - |
dc.contributor.author | Lee, Nam-Taek | - |
dc.contributor.author | Hong, Hyo Jeong | - |
dc.contributor.author | Kim, Yoon-Won | - |
dc.date.accessioned | 2021-09-03T07:44:55Z | - |
dc.date.available | 2021-09-03T07:44:55Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83944 | - |
dc.description.abstract | Organophosphate (OP) nerve agents are known as the most toxic chemical warfare agents that act by inhibiting the enzyme acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Because BuChE is present at a level of about 3,900 times higher than AChE in plasma, most OP agents first react with BuChE in plasma, suggesting that OP-inactivated BuChE (OP-iBuChE) may act as a biomarker of OP exposure. In this study, we generated an anti-BuChE monoclonal antibody (mAb) that has reduced binding activity to VX-inactivated BuChE compared to native BuChE by phage display. We performed subtractive biopanning of a synthetic human Fab library against native BuChE and soman-iBuChE or VX-iBuChE. As the results, we isolated four Fab clones that showed differential binding activities to VX-iBuChE and native BuChE in ELISAs. To confirm the antigen-binding specificity of the selected clones, the Fabs were converted to IgG1s, and the IgG antibodies were expressed in HEK293F cells and purified. One of them (A2) showed approximately 30% reduced binding activity to VX-iBuChE compared to BuChE in a dose-dependent manner, whereas the other three antibodies showed almost the same binding activities to VX-iBuChE and BuChE. In addition, the A2 antibody did not show reduced binding activity to sarin-iBuChE or soman-iBuChE compared to native BuChE. The results indicate that A2 antibody shows reduced binding activity only to VX-iBuChE. A2 antibody may be applied to specific diagnosis of VX exposure. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.subject | CHEMICAL WARFARE AGENTS | - |
dc.subject | NERVE AGENTS | - |
dc.subject | ACETYLCHOLINESTERASE ACTIVITY | - |
dc.subject | BIOLOGICAL SAMPLES | - |
dc.subject | MEDICAL-TREATMENT | - |
dc.subject | ORGANOPHOSPHORUS | - |
dc.subject | EXPOSURE | - |
dc.subject | THERAPEUTICS | - |
dc.subject | TOXICITY | - |
dc.subject | HISTORY | - |
dc.title | Generation of a monoclonal antibody that has reduced binding activity to VX-inactivated butyrylcholinesterase (BuChE) compared to BuChE by phage display | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Nam-Taek | - |
dc.identifier.doi | 10.1007/s12257-017-0110-7 | - |
dc.identifier.scopusid | 2-s2.0-85019203238 | - |
dc.identifier.wosid | 000401323400002 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.22, no.2, pp.114 - 119 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 22 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 114 | - |
dc.citation.endPage | 119 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002222154 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | CHEMICAL WARFARE AGENTS | - |
dc.subject.keywordPlus | NERVE AGENTS | - |
dc.subject.keywordPlus | ACETYLCHOLINESTERASE ACTIVITY | - |
dc.subject.keywordPlus | BIOLOGICAL SAMPLES | - |
dc.subject.keywordPlus | MEDICAL-TREATMENT | - |
dc.subject.keywordPlus | ORGANOPHOSPHORUS | - |
dc.subject.keywordPlus | EXPOSURE | - |
dc.subject.keywordPlus | THERAPEUTICS | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | HISTORY | - |
dc.subject.keywordAuthor | butyrylcholinesterase | - |
dc.subject.keywordAuthor | diagnosis | - |
dc.subject.keywordAuthor | monoclonal antibody | - |
dc.subject.keywordAuthor | nerve toxic agents | - |
dc.subject.keywordAuthor | organophosphate | - |
dc.subject.keywordAuthor | phage display | - |
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