STABILIZED1 Modulates Pre-mRNA Splicing for Thermotolerance
DC Field | Value | Language |
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dc.contributor.author | Kim, Geun-Don | - |
dc.contributor.author | Cho, Young-Hee | - |
dc.contributor.author | Lee, Byeong-Ha | - |
dc.contributor.author | Yoo, Sang-Dong | - |
dc.date.accessioned | 2021-09-03T08:08:54Z | - |
dc.date.available | 2021-09-03T08:08:54Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/84075 | - |
dc.description.abstract | High-temperature stress often leads to differential RNA splicing, thus accumulating different types and/or amounts of mature mRNAs in eukaryotic cells. However, regulatory mechanisms underlying plant precursor mRNA (pre-mRNA) splicing in the environmental stress conditions remain elusive. Herein, we describe that a U5-snRNP-interacting protein homolog STABILIZED1 (STA1) has pre-mRNA splicing activity for heat-inducible transcripts including HEAT STRESS TRANSCRIPTION FACTORs and various HEAT SHOCK PROTEINs for the establishment of heat stress tolerance in Arabidopsis (Arabidopsis thaliana). Our cell-based splicing reporter assay demonstrated STA1 acts on pre-mRNA splicing for specific subsets of stress-related genes. Cellular reconstitution of heat-inducible transcription cascades supported the view that STA1-dependent premRNA splicing plays a role in DREB2A-dependent HSFA3 expression for heat-responsive gene expression. Further genetic analysis with a loss-of-function mutant sta1-1, STA1-expressing transgenic plants in Col background, and STA1-expressing transgenic plants in the sta1-1 background verified that STA1 is essential in expression of necessary genes including HSFA3 for two-step heat stress tolerance in plants. However, constitutive overexpression of the cDNA version of HSFA3 in the sta1-1 background is unable to execute plant heat stress tolerance in sta1-1. Consistently our global target analysis of STA1 showed that its splicing activity modulates a rather broad range of gene expression in response to heat treatment. The findings of this study reveal that heat-inducible STA1 activity for pre-mRNA splicing serves as a molecular regulatory mechanism underlying the plant stress tolerance to high-temperature stress. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC PLANT BIOLOGISTS | - |
dc.subject | HEAT-STRESS RESPONSE | - |
dc.subject | TRANSCRIPTION FACTOR DREB2A | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | COMPLEXITY | - |
dc.subject | PROTEIN | - |
dc.subject | DECAY | - |
dc.subject | STA1 | - |
dc.title | STABILIZED1 Modulates Pre-mRNA Splicing for Thermotolerance | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoo, Sang-Dong | - |
dc.identifier.doi | 10.1104/pp.16.01928 | - |
dc.identifier.scopusid | 2-s2.0-85016515618 | - |
dc.identifier.wosid | 000402054300031 | - |
dc.identifier.bibliographicCitation | PLANT PHYSIOLOGY, v.173, no.4, pp.2370 - 2382 | - |
dc.relation.isPartOf | PLANT PHYSIOLOGY | - |
dc.citation.title | PLANT PHYSIOLOGY | - |
dc.citation.volume | 173 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 2370 | - |
dc.citation.endPage | 2382 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | HEAT-STRESS RESPONSE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR DREB2A | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | COMPLEXITY | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | DECAY | - |
dc.subject.keywordPlus | STA1 | - |
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