Effect of doxycycline on epithelial-mesenchymal transition via the p38/Smad pathway in respiratory epithelial cells
- Authors
- Shin, Jae-Min; Kang, Ju-Hyung; Lee, Seoung-Ae; Park, Il-Ho; Lee, Heung-Man
- Issue Date
- 3월-2017
- Publisher
- OCEAN SIDE PUBLICATIONS INC
- Citation
- AMERICAN JOURNAL OF RHINOLOGY & ALLERGY, v.31, no.2, pp.71 - 77
- Indexed
- SCIE
SCOPUS
- Journal Title
- AMERICAN JOURNAL OF RHINOLOGY & ALLERGY
- Volume
- 31
- Number
- 2
- Start Page
- 71
- End Page
- 77
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/84211
- DOI
- 10.2500/ajra.2017.31.4410
- ISSN
- 1945-8924
- Abstract
- Purpose: Doxycycline has antibacterial and anti-inflammatory effects, and it also suppresses collagen biosynthesis. This study aimed to confirm the effects and mechanism of doxycycline on transforming growth factor (TGF) beta 1 induced epithelial-mesenchymal transition and cell migration in A549 and primary nasal epithelial cells. Methods: A 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide assay and phalloidin-fluorescein isothiocyanate staining were used to evaluate cytotoxicity and cellular morphologic changes. Western blot and immunofluorescence staining were used to determine the expression levels of E-cadherin, vimentin, alpha-smooth muscle actin, fibronectin, phosphorylated Smad2/3, and mitogen-activated protein kinases. Scratch and transwell migration assays were used to assess cellular migration ability. Results: Doxycycline (0-10 mu g/mL) had no significant cytotoxic effects in A549 and primary nasal epithelial cells. Increased expression of mesenchymal markers, including vimentin, alpha-smooth muscle actin, and fibronectin in TGF beta 1 induced A549 cells were downregulated by doxycycline treatment. In contrast, E-cadherin expression was upregulated in TGF beta 1 induced A549 cells. An in vitro cell migration assay showed that doxycycline also inhibited the ability of TGF beta 1 induced migration. Doxycycline treatment suppressed the activation of Smad2/3 and p38, whereas its inhibitory effects were similar to each element-specific inhibitor in A549 and primary nasal epithelial cells. Conclusion: Doxycycline inhibited TGF beta 1 induced epithelial-to-mesenchymal transition and migration by targeting Smad2/3 and p38 signal pathways in respiratory epithelial cells.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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