Assembly of polymer micelles through the sol-gel transition for effective cancer therapy
- Authors
- Ul Khaliq, Nisar; Oh, Keun Sang; Sandra, Febrina Carolina; Joo, Yeonhee; Lee, Juhyung; Byun, Youngro; Kim, In-San; Kwon, Ick Chan; Seo, Jae Hong; Kim, Sang Yoon; Yuk, Soon Hong
- Issue Date
- 10-1월-2017
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Sol-gel transition; Apoptosis; Photodynamic therapy; Prodrug; Pluronic nanoparticles
- Citation
- JOURNAL OF CONTROLLED RELEASE, v.255, pp.258 - 269
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CONTROLLED RELEASE
- Volume
- 255
- Start Page
- 258
- End Page
- 269
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/84932
- DOI
- 10.1016/j.jconrel.2017.04.039
- ISSN
- 0168-3659
- Abstract
- Photo-induced apoptosis-targeted chemotherapy (PIATC) was designed and characterized to propose a new protocol for improved chemotherapy. Intratumoral injection was selected as the mode of administration of the anticancer drug, doxorubicin (DOX). To extend the retention time of DOX at the tumor parenchyma, in-situ gel formation was induced through the sol-gel transition of the Pluronic NPs containing a prodrug of DOX or a photosensitizer. The prodrug (DEVD-S-DOX) was designed to be inactive with a peptide moiety (Aspartic acid-Glutamic acid-Valine-Aspartic acid: DEVD) linked to DOX and to be cleaved into free DOX by caspase-3 expressed with apoptosis. For reactive oxygen species (ROS)-mediated apoptosis, photo-irradiation with methylene blue (MB, photosensitizer) was utilized. The sol-gel transition of the Pluronic NPs containing reactive species, DEVD-S-DOX or MB, was examined by measuring the cloud point and the gel strength in response to temperature change. ROS-mediated apoptosis was observed by measuring the ROS and membrane integrity with induced apoptosis. The in vivo antitumor efficacy of PIATC was measured with a cardiotoxicity assay in tumor-bearing mice.
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Collections - Graduate School > KU-KIST Graduate School of Converging Science and Technology > 1. Journal Articles
- College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
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