Polaribacter vadi sp nov., isolated from a marine gastropod

Abstract

A Gram-reaction-negative, non-motile, yellow-pigmented, NaCl-requiring bacterial strain, designated LPB0003(T), was isolated from a marine gastropod, the granulated dogwhelk (Thais luteostoma). The complete genome sequence of strain LPB0003(T) (GenBank accession number CP017477), sized 3.81 Mb, had 3296 protein-coding genes. 16S rRNA gene sequence analysis indicated that strain LPB0003(T) belongs to the genus Polaribacter within the family Flavobacteriaceae with sequence similarities of 95.3-97.8% to other species of the genus Polaribacter. The average nucleotide identities with closely related species were not higher than 80 %. The isoprenoid quinone (MK-6) and DNA G+C content (29.6 mol%) of strain LPB0003T fell within the expected ranges for the genus Polaribacter. The major polar lipids of the isolate were phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid, an unidentified glycolipid, and two unidentified lipids. The predominant cellular fatty acids were iso-C-15:0, iso-C-15:0 3-OH, C-16:1 omega 7c and/or C-16:1 omega 6c (summed feature 3), and C-15:1 omega 6c. Based on phylogenetic, genomic and phenotypic data presented in this study, strain LPB0003(T) should be classified as a novel species of the genus Polaribacter, for which the name Polaribacter vadi sp. nov. is proposed. The type strain is LPB0003(T) (= KACC 18704(T) = JCM 31217(T)).