Utilization of algal sugars and glycerol for enhanced cephalosporin C production by Acremonium chrysogenum M35

Abstract

In our previous study, glycerol was utilized as an additional carbon source for the production of cephalosporin C (CPC) by Acremonium chrysogenum M35. In this study, algal sugars extracted from the third-generation biomass were utilized in the CPC production for the first time. The CPC production improved about twofold when using the algal sugars as the carbon source. The complex medium including algal sugars and glycerol was utilized, and 7<bold></bold>3gl(-1) CPC production was achieved in a 250-ml shaking flask. To determine the important variables for the CPC production, Plackett-Burman design was carried out and 6<bold></bold>18gl(-1) of CPC was estimated under the numerically optimized conditions. Under the optimized conditions, the CPC production was performed in a 5-l scale bioreactor, affording CPC production at a rate of 7<bold></bold>1gl(-1). Moreover, 6<bold></bold>7gl(-1) CPC was produced using crude glycerol as the substrate.