Identification of molecular markers distinguishing adult neural stem cells in the subventricular and subcallosal zones
- Authors
- Kim, Joo Yeon; Shaker, Mohammed R.; Lee, Ju-Hyun; Lee, Boram; Kim, Hyun; Sun, Woong
- Issue Date
- 2017
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Subventricular zone; subcallosal zone; adult neural stem cell; CRBP1; HMGA1; ZIC2; microarray
- Citation
- ANIMAL CELLS AND SYSTEMS, v.21, no.3, pp.152 - 159
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ANIMAL CELLS AND SYSTEMS
- Volume
- 21
- Number
- 3
- Start Page
- 152
- End Page
- 159
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86283
- DOI
- 10.1080/19768354.2017.1324522
- ISSN
- 1976-8354
- Abstract
- Neural stem cells (NSCs) in the adult subventricular zone (SVZ) are regionally specified and have distinct molecular gene expression signatures. Recently, we identified the subcallosal zone (SCZ) as a novel brain region where adult NSCs maintain and spontaneously produce neuroblasts. In an attempt to isolate genes specifically expressed in the SCZ or SVZ, microarray analyses of their differentially expressing transcripts were done. The comparison between neurospheres generated from SVZ and SCZ revealed differential expression >1.5-fold in two groups in only 83 genes, representing <0.03% of the genes examined, suggesting that these two populations are largely similar. The differential expression patterns SCZ and SVZ genes were confirmed by RT-PCR and Western blots. The selective expressions of two genes (CRBP1, HMGA1) in SVZ-NSCs were further confirmed by immunohistochemistry. These molecular markers could be useful for further molecular and cellular characterization of NSCs.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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