MG53-IRS-1 (Mitsugumin 53-Insulin Receptor Substrate-1) Interaction Disruptor Sensitizes Insulin Signaling in Skeletal Muscle
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyun | - |
dc.contributor.author | Park, Jung-Jin | - |
dc.contributor.author | Nga Nguyen | - |
dc.contributor.author | Park, Jun Sub | - |
dc.contributor.author | Hong, Jin | - |
dc.contributor.author | Kim, Seung-Hyeob | - |
dc.contributor.author | Song, Woon Young | - |
dc.contributor.author | Kim, Hak Joong | - |
dc.contributor.author | Choi, Kwangman | - |
dc.contributor.author | Cho, Sungchan | - |
dc.contributor.author | Lee, Jae-Seon | - |
dc.contributor.author | Kim, Bong-Woo | - |
dc.contributor.author | Ko, Young-Gyu | - |
dc.date.accessioned | 2021-09-03T15:36:05Z | - |
dc.date.available | 2021-09-03T15:36:05Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-12-23 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/86500 | - |
dc.description.abstract | Mitsugumin 53 (MG53) is an E3 ligase that interacts with and ubiquitinates insulin receptor substrate-1 (IRS-1) in skeletal muscle; thus, an MG53-IRS-1 interaction disruptor (MID), which potentially sensitizes insulin signaling with an elevated level of IRS-1 in skeletal muscle, is an excellent candidate for treating insulin resistance. To screen for an MID, we developed a bimolecular luminescence complementation system using an N-terminal luciferase fragment fused with IRS-1 and a C-terminal luciferase fragment fused with an MG53 C14A mutant that binds to IRS-1 but does not have E3 ligase activity. An MID, which was discovered using the bimolecular luminescence complementation system, disrupted the molecular association of MG53 with IRS-1, thus abolishing MG53-mediated IRS-1 ubiquitination and degradation. Thus, the MID sensitized insulin signaling and increased insulin-elicited glucose uptake with an elevated level of IRS-1 in C2C12 myotubes. These data indicate that this MID holds promise as a drug candidate for treating insulin resistance. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | MEMBRANE REPAIR | - |
dc.subject | MUSCULAR-DYSTROPHY | - |
dc.subject | MG53 | - |
dc.subject | INJURY | - |
dc.subject | MYOGENESIS | - |
dc.subject | OBESITY | - |
dc.subject | KINASE | - |
dc.subject | TRIM72 | - |
dc.title | MG53-IRS-1 (Mitsugumin 53-Insulin Receptor Substrate-1) Interaction Disruptor Sensitizes Insulin Signaling in Skeletal Muscle | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hak Joong | - |
dc.contributor.affiliatedAuthor | Kim, Bong-Woo | - |
dc.contributor.affiliatedAuthor | Ko, Young-Gyu | - |
dc.identifier.doi | 10.1074/jbc.M116.754424 | - |
dc.identifier.scopusid | 2-s2.0-85007310688 | - |
dc.identifier.wosid | 000391571400004 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.291, no.52, pp.26627 - 26635 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 291 | - |
dc.citation.number | 52 | - |
dc.citation.startPage | 26627 | - |
dc.citation.endPage | 26635 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | MEMBRANE REPAIR | - |
dc.subject.keywordPlus | MUSCULAR-DYSTROPHY | - |
dc.subject.keywordPlus | MG53 | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | MYOGENESIS | - |
dc.subject.keywordPlus | OBESITY | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | TRIM72 | - |
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