Morphologic Changes of Zebrafish Melanophore after Intense Pulsed Light and Q-Switched Nd:YAG Laser Irradiation
DC Field | Value | Language |
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dc.contributor.author | Ryu, Hwa Jung | - |
dc.contributor.author | Lee, Ji Min | - |
dc.contributor.author | Jang, Hee Won | - |
dc.contributor.author | Park, Hae Chul | - |
dc.contributor.author | Rhyu, Im Joo | - |
dc.contributor.author | Kim, Il-Hwan | - |
dc.date.accessioned | 2021-09-03T16:04:12Z | - |
dc.date.available | 2021-09-03T16:04:12Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-12 | - |
dc.identifier.issn | 1013-9087 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/86601 | - |
dc.description.abstract | Background: Recently, the pulse-in-pulse mode of intense pulsed light (IPL) has been used increasingly for the treatment of melasma. Objective: To observe the morphologic changes in the melanophore in adult zebrafish after irradiation with conventional and pulse-in-pulse IPL and Q-switched Nd:YAG (QSNY) laser. Methods: Adult zebrafish were irradiated with conventional and pulse-in-pulse mode of IPL. The conditions for conventional IPL were 3 mJ/cm(2), 560 nm filter, and pulse widths of 7, 20, and 35 msec. The pulse-in-pulse conditions were 3 mJ/cm(2) and on-time 1/off-time 2. The QSNY laser was used with the settings of 1,064 nm, 0.4J/cm(2), a 7 mm spot size, and one shot. Specimens were observed using a light microscope, a transmission electron microscope (TEM), a scanning electron microscope (SEM) and a confocal microscope. Results: After conventional IPL irradiation with a 7 msec pulse width, melanophore breakage was observed using light microscopy. Under TEM, irradiation with conventional IPL for 7 msec and pulse-in-pulse IPL induced melanophore thermolysis with vacuolization. However, changes in the melanophore were not observed with 35 msec IPL. Under SEM, unlike the control and QSNY groups, IPL-irradiated zebrafish showed finger -like fusion in the protein structure of scales. Specimens examined by a confocal microscope after conventional IPL irradiation showed a larger green-stained area on TUNEL staining than that after pulse-in-pulse mode IPL irradiation. Conclusion: Zebrafish irradiated with long pulse-IPL showed no morphologic changes using light microscopy, while morphological changes in melanophores were evident with use of TEM. Pulse-in-pulse mode IPL caused less damage than conventional IPL. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN DERMATOLOGICAL ASSOC | - |
dc.subject | SELECTIVE PHOTOTHERMOLYSIS | - |
dc.subject | MELASMA TREATMENT | - |
dc.subject | PIGMENTATION | - |
dc.title | Morphologic Changes of Zebrafish Melanophore after Intense Pulsed Light and Q-Switched Nd:YAG Laser Irradiation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ryu, Hwa Jung | - |
dc.contributor.affiliatedAuthor | Park, Hae Chul | - |
dc.contributor.affiliatedAuthor | Rhyu, Im Joo | - |
dc.contributor.affiliatedAuthor | Kim, Il-Hwan | - |
dc.identifier.doi | 10.5021/ad.2016.28.6.711 | - |
dc.identifier.scopusid | 2-s2.0-85000417150 | - |
dc.identifier.wosid | 000397356100006 | - |
dc.identifier.bibliographicCitation | ANNALS OF DERMATOLOGY, v.28, no.6, pp.711 - 717 | - |
dc.relation.isPartOf | ANNALS OF DERMATOLOGY | - |
dc.citation.title | ANNALS OF DERMATOLOGY | - |
dc.citation.volume | 28 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 711 | - |
dc.citation.endPage | 717 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002169965 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Dermatology | - |
dc.relation.journalWebOfScienceCategory | Dermatology | - |
dc.subject.keywordPlus | SELECTIVE PHOTOTHERMOLYSIS | - |
dc.subject.keywordPlus | MELASMA TREATMENT | - |
dc.subject.keywordPlus | PIGMENTATION | - |
dc.subject.keywordAuthor | Intense pulsed light | - |
dc.subject.keywordAuthor | Melanophores | - |
dc.subject.keywordAuthor | Zebrafish | - |
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