Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen
DC Field | Value | Language |
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dc.contributor.author | Ha, Na-Young | - |
dc.contributor.author | Shin, Hyun Mu | - |
dc.contributor.author | Sharma, Prashant | - |
dc.contributor.author | Cho, Hyun Ah | - |
dc.contributor.author | Min, Chan-Ki | - |
dc.contributor.author | Kim, Hong-il | - |
dc.contributor.author | Nguyen Thi Hai Yen | - |
dc.contributor.author | Kang, Jae-Seung | - |
dc.contributor.author | Kim, Ik-Sang | - |
dc.contributor.author | Choi, Myung-Sik | - |
dc.contributor.author | Kim, Young Keun | - |
dc.contributor.author | Cho, Nam-Hyuk | - |
dc.date.accessioned | 2021-09-03T16:44:09Z | - |
dc.date.available | 2021-09-03T16:44:09Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-11-26 | - |
dc.identifier.issn | 1477-3155 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/86799 | - |
dc.description.abstract | Background: Zinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP. Results: A novel zinc oxide-binding peptide (ZBP), FPYPGGDA, with high affinity to ZNP (K-a = 2.26 x 10(6) M-1) was isolated from a random peptide library and fused with a bacterial antigen, ScaA of Orientia tsutsugamushi, the causative agent of scrub typhus. The ZNP/ZBP-ScaA complex was efficiently phagocytosed by a dendritic cell line, DC2.4, in vitro and significantly enhanced anti-ScaA antibody responses in vivo compared to control groups. In addition, immunization with the ZNP/ZBP-ScaA complex promoted the generation of IFN-gamma-secreting T cells in an antigen-dependent manner. Finally, we observed that ZNP/ZBP-ScaA immunization provided protective immunity against lethal challenge of O. tsutsugamushi, indicating that ZNP can be used as a potent adjuvant when complexed with ZBP-conjugated antigen. Conclusions: ZNPs possess good adjuvant potential as a vaccine carrier when combined with an antigen having a high affinity to ZNP. When complexed with ZBP-ScaA antigen, ZNPs could induce strong antibody responses as well as protective immunity against lethal challenges of O. tsutsugamushi. Therefore, application of ZNPs combined with a specific soluble antigen could be a promising strategy as a novel vaccine carrier system. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.subject | SCRUB TYPHUS | - |
dc.subject | IN-VITRO | - |
dc.subject | ZNO NANOPARTICLES | - |
dc.subject | BINDING PEPTIDES | - |
dc.subject | TOXICITY | - |
dc.subject | CELLS | - |
dc.subject | MICE | - |
dc.subject | MECHANISMS | - |
dc.subject | RESPONSES | - |
dc.subject | PROTEINS | - |
dc.title | Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young Keun | - |
dc.identifier.doi | 10.1186/s12951-016-0229-2 | - |
dc.identifier.scopusid | 2-s2.0-85005978160 | - |
dc.identifier.wosid | 000389281700001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOBIOTECHNOLOGY, v.14 | - |
dc.relation.isPartOf | JOURNAL OF NANOBIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF NANOBIOTECHNOLOGY | - |
dc.citation.volume | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | SCRUB TYPHUS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ZNO NANOPARTICLES | - |
dc.subject.keywordPlus | BINDING PEPTIDES | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordAuthor | Zinc oxide nanoparticle | - |
dc.subject.keywordAuthor | ZnO binding peptide | - |
dc.subject.keywordAuthor | Scrub typhus | - |
dc.subject.keywordAuthor | Vaccine | - |
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