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Sensitivity improvement of an electrical sensor achieved by control of biomolecules based on the negative dielectrophoretic force

Authors
Kim, Hye JinKim, JinsikYoo, Yong KyoungLee, Jeong HoonPark, Jung HoHwang, Kyo Seon
Issue Date
15-11월-2016
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Keywords
Sensitivity; Limit of detection; Dielectrophoresis; Electrical sensor; Immunochemical method
Citation
BIOSENSORS & BIOELECTRONICS, v.85, pp.977 - 985
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS & BIOELECTRONICS
Volume
85
Start Page
977
End Page
985
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/86827
DOI
10.1016/j.bios.2016.06.081
ISSN
0956-5663
Abstract
Effective control of nano-scale biomolecules can enhance the sensitivity and limit of detection of an interdigitated microelectrode (IME) sensor. Manipulation of the biomolecules by dielectrophoresis (DEP), especially the negative DEP (nDEP) force, so that they are trapped between electrodes (sensing regions) was predicted to increase the binding efficiency of the antibody and target molecules, leading to a more effective reaction. To prove this concept, amyloid beta 42 (Af342) and prostate specific antigen (PSA) protein were respectively trapped between the sensing region owing to the nDEP force under 5 V and 0.05 V, which was verified with COMSOL simulation. Using the simulation value, the resistance change (AR/Rb) of the IME sensor from the specific antibody-antigen reaction of the two biomolecules and the change in fluorescence intensity were compared in the reference (pDEP) and nDEP conditions. The AR/Rb value improved by about 2-fold and 1.66-fold with nDEP compared to the reference condition with various protein concentrations, and these increases were confirmed with fluorescence imaging. Overall, nDEP enhanced the detection sensitivity for A beta(42) and PSA by 128% and 258%, respectively, and the limit of detection improved by up to 2-orders of magnitude. These results prove that DEP can improve the biosensor's performance. (C) 2016 Elsevier B.V. All rights reserved.
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