Precise precursor rebalancing for isoprenoids production by fine control of gapA expression in Escherichia coli
DC Field | Value | Language |
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dc.contributor.author | Jung, Juyoung | - |
dc.contributor.author | Lim, Jae Hyung | - |
dc.contributor.author | Kim, Se Yeon | - |
dc.contributor.author | Im, Dae-Kyun | - |
dc.contributor.author | Seok, Joo Yeon | - |
dc.contributor.author | Lee, Seung-Jae V. | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.contributor.author | Jung, Gyoo Yeol | - |
dc.date.accessioned | 2021-09-03T17:20:49Z | - |
dc.date.available | 2021-09-03T17:20:49Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-11 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/86938 | - |
dc.description.abstract | Biosynthesis of isoprenoids via the 1-deoxy-D-xylulose-5-phosphate (DXP) pathway requires equimolar glyceraldehyde 3-phosphate and pyruvate to divert carbon flux toward the products of interest. Here, we demonstrate that precursor balancing is one of the critical steps for the production of isoprenoids in Escherichia coll. First, the implementation of the synthetic lycopene production pathway as a model system and the amplification of the native DXP pathway were accomplished using synthetic constitutive promoters and redesigned 5'-untranslated regions (5'-UTRs). Next, fine-controlled precursor balancing was investigated by tuning phosphoenolpyruvate synthase (PpsA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The results showed that tuning-down of gapA improved the specific lycopene content by 45% compared to the overexpression of ppsA. The specific lycopene content in the strains with down-regulated gapA increased by 97% compared to that in the parental strain. Our results indicate that gapA is the best target for precursor balancing to increase biosynthesis of isoprenoids. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | PATHWAY OPTIMIZATION | - |
dc.subject | GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE | - |
dc.subject | CAROTENOID PRODUCTION | - |
dc.subject | LYCOPENE PRODUCTION | - |
dc.subject | N-BUTANOL | - |
dc.subject | DESIGN | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | GLUCOSE | - |
dc.subject | STRAIN | - |
dc.subject | REGENERATION | - |
dc.title | Precise precursor rebalancing for isoprenoids production by fine control of gapA expression in Escherichia coli | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1016/j.ymben.2016.10.003 | - |
dc.identifier.scopusid | 2-s2.0-84995437675 | - |
dc.identifier.wosid | 000387984600039 | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.38, pp.401 - 408 | - |
dc.relation.isPartOf | METABOLIC ENGINEERING | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 38 | - |
dc.citation.startPage | 401 | - |
dc.citation.endPage | 408 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | PATHWAY OPTIMIZATION | - |
dc.subject.keywordPlus | GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE | - |
dc.subject.keywordPlus | CAROTENOID PRODUCTION | - |
dc.subject.keywordPlus | LYCOPENE PRODUCTION | - |
dc.subject.keywordPlus | N-BUTANOL | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | STRAIN | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordAuthor | Precursor balancing | - |
dc.subject.keywordAuthor | Isoprenoid | - |
dc.subject.keywordAuthor | Lycopene | - |
dc.subject.keywordAuthor | gapA | - |
dc.subject.keywordAuthor | ppsA | - |
dc.subject.keywordAuthor | G3P | - |
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