Comprehensive Proteome Profiling of Platelet Identified a Protein Profile Predictive of Responses to An Antiplatelet Agent Sarpogrelate
- Authors
- Lee, Hangyeore; Chae, Sehyun; Park, Jisook; Bae, Jingi; Go, Eun-Bi; Kim, Su-Jin; Kim, Hokeun; Hwang, Daehee; Lee, Sang-Won; Lee, Soo-Youn
- Issue Date
- 11월-2016
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Citation
- MOLECULAR & CELLULAR PROTEOMICS, v.15, no.11, pp.3461 - 3472
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR & CELLULAR PROTEOMICS
- Volume
- 15
- Number
- 11
- Start Page
- 3461
- End Page
- 3472
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86991
- DOI
- 10.1074/mcp.M116.059154
- ISSN
- 1535-9476
- Abstract
- Sarpogrelate is an antiplatelet agent widely used to treat arterial occlusive diseases. Evaluation of platelet aggregation is essential to monitor therapeutic effects of sarpogrelate. Currently, no molecular signatures are available to evaluate platelet aggregation. Here, we performed comprehensive proteome profiling of platelets collected from 18 subjects before and after sarpogrelate administration using LC-MS/MS analysis coupled with extensive fractionation. Of 5423 proteins detected, we identified 499 proteins affected by sarpogrelate and found that they strongly represented cellular processes related to platelet activation and aggregation, including cell activation, coagulation, and vesicle-mediated transports. Based on the network model of the proteins involved in these processes, we selected three proteins (cut-like homeobox 1; coagulation factor XIII, B polypeptide; and peptidylprolyl isomerase D) that reflect the platelet aggregation-related processes after confirming their alterations by sarpogrelate in independent samples using Western blotting. Our proteomic approach provided a protein profile predictive of therapeutic effects of sarpogrelate.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Science > Department of Chemistry > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.