Non-deep simple morphophysiological dormancy in seeds of heavenly bamboo (Nandina domestica Thunb.)

Abstract

Heavenly bamboo (Nandina domestica) is a popular ornamental shrub used as an indoor plant and for landscaping. However, seed propagation has been limited due to its very slow germination (9 months under natural conditions) caused by an extended seed dormancy period. We used various temperature treatments, including simulations of seasonal temperature changes, to investigate the temperature requirements for dormancy breaking and the germination of heavenly bamboo seeds at the embryo and whole-seed levels. Heavenly bamboo seeds were found to have no physical dormancy because the seeds imbibed water readily. However, its mature seeds contained underdeveloped embryos (E:S ratio <0.2) that had morphological dormancy. The seeds did not germinate within a month of incubation at fluctuating temperatures of 25/15, 20/10, and 15/6, or at 5 degrees C alone, which indicated that they had morphophysiological dormancy as well as morphological dormancy. A simulation study of seasonal temperatures (move-along test) showed that warm stratification (25/15 degrees C) was needed to stimulate embryo growth and germinate the seeds, but cold stratification (5 degrees C) was not required. Warm stratification at a constant 20 degrees C hastened seed germination more than a fluctuating temperature at 25/15 degrees C, and it led to a germination time that was shorter by 4 months than that under natural conditions. Application of gibberellic acid (GA(3)) at 100 and 1000 mgL(-1) could substitute for warm stratification and broke dormancy in seeds incubated at 15/6 degrees C. These results suggested that heavenly bamboo seeds had non-deep simple morphophysiological dormancy, which could be broken by warm stratification at 25/15 or 20 degrees C. (C) 2016 Elsevier B.V. All rights reserved.