Enhanced butanol fermentation using metabolically engineered Clostridium acetobutylicum with ex situ recovery of butanol
DC Field | Value | Language |
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dc.contributor.author | Lee, Sang-Hyun | - |
dc.contributor.author | Kim, Sooah | - |
dc.contributor.author | Kim, Jung Yeon | - |
dc.contributor.author | Cheong, Nam Yong | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.date.accessioned | 2021-09-03T18:59:41Z | - |
dc.date.available | 2021-09-03T18:59:41Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/87240 | - |
dc.description.abstract | In this study, metabolic target reactions for strain engineering were searched via intracellular coenzyme A (CoA) metabolite analysis. The metabolic reactions catalyzed by thiolase (AtoB) and aldehyde-alcohol dehydrogenase (AdhE1) were considered potential rate-limiting steps. In addition, CoA transferase (CtfAB) was highlighted as being important for the assimilation of organic acids, in order to achieve high butanol production. Based on this quantitative analysis, the BEKW_E1AB-atoB strain was constructed by overexpressing the thl (atoB), adhE1, and ctfAB genes in Clostridium acetobutylicum strain BEKW, which has the phosphotransacetylase (pta) and butyrate kinase (buk) genes knocked out. After 100 h of continuous fermentation coupled with adsorptive ex situ butanol recovery, the concentrations found after considering desorption, yield, and productivity for the BEKW_E1AB-atoB strain were 55.7 g/L, 0.38 g/g, and 2.64 g/L/h, respectively. The level of butanol production achieved (2.64 g/L/h) represents the highest reported value obtained after adsorptive, long-term fermentation. (C) 2016 ElsevierLtd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | BEIJERINCKII NCIMB 8052 | - |
dc.subject | GENOME-SCALE MODEL | - |
dc.subject | PRODUCT RECOVERY | - |
dc.subject | ADSORPTION | - |
dc.subject | SOLVENTOGENESIS | - |
dc.subject | SEQUENCE | - |
dc.subject | NETWORK | - |
dc.subject | CELLULOLYTICUM | - |
dc.subject | RECONSTRUCTION | - |
dc.subject | ACIDOGENESIS | - |
dc.title | Enhanced butanol fermentation using metabolically engineered Clostridium acetobutylicum with ex situ recovery of butanol | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kyoung Heon | - |
dc.identifier.doi | 10.1016/j.biortech.2016.07.060 | - |
dc.identifier.scopusid | 2-s2.0-84978370478 | - |
dc.identifier.wosid | 000384710500116 | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.218, pp.909 - 917 | - |
dc.relation.isPartOf | BIORESOURCE TECHNOLOGY | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 218 | - |
dc.citation.startPage | 909 | - |
dc.citation.endPage | 917 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.subject.keywordPlus | BEIJERINCKII NCIMB 8052 | - |
dc.subject.keywordPlus | GENOME-SCALE MODEL | - |
dc.subject.keywordPlus | PRODUCT RECOVERY | - |
dc.subject.keywordPlus | ADSORPTION | - |
dc.subject.keywordPlus | SOLVENTOGENESIS | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | NETWORK | - |
dc.subject.keywordPlus | CELLULOLYTICUM | - |
dc.subject.keywordPlus | RECONSTRUCTION | - |
dc.subject.keywordPlus | ACIDOGENESIS | - |
dc.subject.keywordAuthor | Acetone-butanol-ethanol fermentation | - |
dc.subject.keywordAuthor | Clostridium acetobutylicum | - |
dc.subject.keywordAuthor | Coenzyme A | - |
dc.subject.keywordAuthor | Intracellular metabolite analysis | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
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