Toll-like receptor 4-mediated expression of interleukin-32 via the c-Jun N-terminal kinase/protein kinase B/cyclic adenosine monophosphate response element binding protein pathway in chronic rhinosinusitis with nasal polyps
DC Field | Value | Language |
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dc.contributor.author | Cho, Jung-Sun | - |
dc.contributor.author | Kim, Jin-Ah | - |
dc.contributor.author | Park, Joo-Hoo | - |
dc.contributor.author | Park, Il-Ho | - |
dc.contributor.author | Han, In-Hye | - |
dc.contributor.author | Lee, Heung-Man | - |
dc.date.accessioned | 2021-09-03T19:21:14Z | - |
dc.date.available | 2021-09-03T19:21:14Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 2042-6976 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/87344 | - |
dc.description.abstract | Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is prolonged inflammation of the sinonasal mucosa. Interleukin-32 (IL-32) is involved in the pathogenesis of chronic lung inflammatory diseases. The aim of study is to compare the expression level of IL-32 in normal nasal mucosa and CRSwNP and to investigate the mechanism underlying IL-32 expression in CRSwNP. Methods: IL-32 expression in nasal tissues, normal nasal mucosa-derived fibroblasts (NorDFs) and nasal polypderived fibroblasts (NPDFs), ex vivo explants of nasal tissues was measured by reverse-transcription polymerase chain reaction (RT-PCR), Western blotting, and enzyme-linked immunosorbent assay (ELISA). NorDFs and NPDFs were exposed to lipopolysaccharide (LPS) and the expression level of IL-32 was measured. LPS from Rhodobactersphaeroides (RS) and small interference RNA against Toll-like receptor 4 (siTLR4) were used to inhibit signaling by TLR4. Activation ofmitogen-activated protein kinase (MAPKs) (extracellular related kinase [ERK], p38, and c-Jun N-terminal kinase [JNK]), protein kinase B (AKT), and cyclic adenosine monophosphate response element binding protein (CREB) was examined using western blot analysis. Results: Expression of IL-32 was increased in CRSwNP compared to normal nasal mucosa. LPS induced expression of IL-32 in a time-dependent manner. The in-duction of IL-32 expression in NPDFS was more effective than in NorDFs. Treatment with RS and siTLR4 inhibited the messenger RNA (mRNA) expression of TLR4, myeloid differentiation primary response 88 (MyD88), and IL-32 in LPS-stimulated NPDFs. IL-32 expression was specifically activated by JNK, AKT, and CREB in LPS-stimulated NPDFs and CRSwNP ex vivo explants. Conclusion: The sensitivity for IL-32 expression by LPS was increased in CRSwNP compared to normal nasal mucosa. LPS effectively induced IL-32 expression in NPDFs than in NorDFs through the TLR4-JNK-AKT-CREB signaling pathway. Therefore, IL-32 seems to be involved in the pathogenesis of CRSwNP. (C) 2016 ARS-AAOA, LLC. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | KAPPA-B | - |
dc.subject | IL-32 | - |
dc.subject | CYTOKINE | - |
dc.subject | INHIBITION | - |
dc.subject | CELLS | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | IL-1-BETA | - |
dc.subject | MECHANISM | - |
dc.subject | DISEASE | - |
dc.title | Toll-like receptor 4-mediated expression of interleukin-32 via the c-Jun N-terminal kinase/protein kinase B/cyclic adenosine monophosphate response element binding protein pathway in chronic rhinosinusitis with nasal polyps | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Il-Ho | - |
dc.contributor.affiliatedAuthor | Lee, Heung-Man | - |
dc.identifier.doi | 10.1002/alr.21792 | - |
dc.identifier.scopusid | 2-s2.0-85027928406 | - |
dc.identifier.wosid | 000388309600004 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY, v.6, no.10, pp.1020 - 1028 | - |
dc.relation.isPartOf | INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY | - |
dc.citation.title | INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY | - |
dc.citation.volume | 6 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1020 | - |
dc.citation.endPage | 1028 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Otorhinolaryngology | - |
dc.relation.journalWebOfScienceCategory | Otorhinolaryngology | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | IL-32 | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | IL-1-BETA | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordAuthor | chronic rhinosinusitis | - |
dc.subject.keywordAuthor | nasal polyp | - |
dc.subject.keywordAuthor | Toll-like receptor | - |
dc.subject.keywordAuthor | interleukin-32 | - |
dc.subject.keywordAuthor | fibroblast | - |
dc.subject.keywordAuthor | ex vivo explant | - |
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