miR-5003-3p promotes epithelial-mesenchymal transition in breast cancer cells through Snail stabilization and direct targeting of E-cadherin
DC Field | Value | Language |
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dc.contributor.author | Kwak, Seo-Young | - |
dc.contributor.author | Yoo, Je-Ok | - |
dc.contributor.author | An, Hyun-Ju | - |
dc.contributor.author | Bae, In-Hwa | - |
dc.contributor.author | Park, Myung-Jin | - |
dc.contributor.author | Kim, Joon | - |
dc.contributor.author | Han, Young-Hoon | - |
dc.date.accessioned | 2021-09-03T19:22:10Z | - |
dc.date.available | 2021-09-03T19:22:10Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 1674-2788 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/87349 | - |
dc.description.abstract | One of the initial steps in metastatic dissemination is the epithelial-mesenchymal transition (EMT). Along this line, microRNAs (miRNAs) have been shown to function as important regulators of tumor progression at various stages. Therefore, we performed a functional screening for EMT-regulating miRNAs and identified several candidate miRNAs. Among these, we demonstrated that miR-5003-3p induces cellular features characteristic of EMT. miR-5003-3p induced upregulation of Snail, a key EMT-promoting transcription factor and transcriptional repressor of E-cadherin, through protein stabilization. MDM2 was identified as a direct target of miR-5003-3p, the downregulation of which induced Snail stabilization. E-cadherin was also demonstrated to be a direct target of miR-5003-3p, reinforcing the EMT-promoting function of miR-5003-3p. In situ hybridization and immunohistochemical analyses using tissue microarrays revealed that miR-5003-3p expression was higher in paired metastatic breast carcinoma tissues than in primary ductal carcinoma tissues, and was inversely correlated with the expression of MDM2 and E-cadherin. Furthermore, miR-5003-3p enhanced the formation of metastatic nodules in the lungs of mice in a tail vein injection experiment. Collectively, our results suggest that miR-5003-3p functions as a metastasis activator by promoting EMT through dual regulation of Snail stability and E-cadherin, and may therefore be a potential therapeutic target in metastatic cancers. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | TRANSCRIPTION FACTORS | - |
dc.subject | TUMOR-SUPPRESSOR | - |
dc.subject | METASTASIS | - |
dc.subject | MICRORNA | - |
dc.subject | INVASION | - |
dc.subject | MIR-200 | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | DEGRADATION | - |
dc.subject | CARCINOMA | - |
dc.subject | REVEALS | - |
dc.title | miR-5003-3p promotes epithelial-mesenchymal transition in breast cancer cells through Snail stabilization and direct targeting of E-cadherin | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Joon | - |
dc.identifier.doi | 10.1093/jmcb/mjw026 | - |
dc.identifier.scopusid | 2-s2.0-84992390971 | - |
dc.identifier.wosid | 000386455300002 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MOLECULAR CELL BIOLOGY, v.8, no.5, pp.372 - 383 | - |
dc.relation.isPartOf | JOURNAL OF MOLECULAR CELL BIOLOGY | - |
dc.citation.title | JOURNAL OF MOLECULAR CELL BIOLOGY | - |
dc.citation.volume | 8 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 372 | - |
dc.citation.endPage | 383 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTORS | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR | - |
dc.subject.keywordPlus | METASTASIS | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordPlus | MIR-200 | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordAuthor | miR-5003-3p | - |
dc.subject.keywordAuthor | EMT | - |
dc.subject.keywordAuthor | metastasis | - |
dc.subject.keywordAuthor | E-cadherin | - |
dc.subject.keywordAuthor | Snail | - |
dc.subject.keywordAuthor | MDM2 | - |
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