Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Sun Young | - |
dc.contributor.author | Lee, Hyun Jeong | - |
dc.contributor.author | Choi, Jaeyeon | - |
dc.contributor.author | Kim, Jiye | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.contributor.author | Um, Youngsoon | - |
dc.contributor.author | Kim, Yunje | - |
dc.contributor.author | Lee, Taek Soon | - |
dc.contributor.author | Keasling, Jay D. | - |
dc.contributor.author | Woo, Han Min | - |
dc.date.accessioned | 2021-09-03T19:57:04Z | - |
dc.date.available | 2021-09-03T19:57:04Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-09-22 | - |
dc.identifier.issn | 1754-6834 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/87491 | - |
dc.description.abstract | Background: Metabolic engineering of cyanobacteria has enabled photosynthetic conversion of CO2 to value-added chemicals as bio-solar cell factories. However, the production levels of isoprenoids in engineered cyanobacteria were quite low, compared to other microbial hosts. Therefore, modular optimization of multiple gene expressions for metabolic engineering of cyanobacteria is required for the production of farnesyl diphosphate-derived isoprenoids from CO2. Results: Here, we engineered Synechococcus elongatus PCC 7942 with modular metabolic pathways consisting of the methylerythritol phosphate pathway enzymes and the amorphadiene synthase for production of amorpha-4,11-di-ene, resulting in significantly increased levels (23-fold) of amorpha-4,11-diene (19.8 mg/L) in the best strain relative to a parental strain. Replacing amorphadiene synthase with squalene synthase led to the synthesis of a high amount of squalene (4.98 mg/L/OD730). Overexpression of farnesyl diphosphate synthase is the most critical factor for the significant production, whereas overexpression of 1-deoxy-d-xylulose 5-phosphate reductase is detrimental to the cell growth and the production. Additionally, the cyanobacterial growth inhibition was alleviated by expressing a terpene synthase in S. elongatus PCC 7942 strain with the optimized MEP pathway only (SeHL33). Conclusions: This is the first demonstration of photosynthetic production of amorpha-4,11-diene from CO2 in cyanobacteria and production of squalene in S. elongatus PCC 7942. Our optimized modular OverMEP strain (SeHL33) with either co-expression of ADS or SQS demonstrated the highest production levels of amorpha-4,11-diene and squalene, which could expand the list of farnesyl diphosphate-derived isoprenoids from CO2 as bio-solar cell factories. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | MONOTERPENE HYDROCARBONS PRODUCTION | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | ANTIMALARIAL-DRUG | - |
dc.subject | ISOPRENOID PATHWAY | - |
dc.subject | CARBON-DIOXIDE | - |
dc.subject | SYNTHASE | - |
dc.subject | EXPRESSION | - |
dc.subject | ACID | - |
dc.subject | REDUCTOISOMERASE | - |
dc.subject | OPTIMIZATION | - |
dc.title | Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1186/s13068-016-0617-8 | - |
dc.identifier.scopusid | 2-s2.0-85009121364 | - |
dc.identifier.wosid | 000383751300001 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY FOR BIOFUELS, v.9 | - |
dc.relation.isPartOf | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.title | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.volume | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.subject.keywordPlus | MONOTERPENE HYDROCARBONS PRODUCTION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | ANTIMALARIAL-DRUG | - |
dc.subject.keywordPlus | ISOPRENOID PATHWAY | - |
dc.subject.keywordPlus | CARBON-DIOXIDE | - |
dc.subject.keywordPlus | SYNTHASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | REDUCTOISOMERASE | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Cyanobacteria | - |
dc.subject.keywordAuthor | Synthetic biology | - |
dc.subject.keywordAuthor | Isoprenoids | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.