Detection of airborne bacteria with disposable bio-precipitator and NanoGene assay
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Eun-Hee | - |
dc.contributor.author | Chua, Beelee | - |
dc.contributor.author | Son, Ahjeong | - |
dc.date.accessioned | 2021-09-03T19:59:51Z | - |
dc.date.available | 2021-09-03T19:59:51Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-09-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/87505 | - |
dc.description.abstract | We demonstrated the detection of airborne bacteria by a disposable bio-precipitator and NanoGene assay combination. The bio-precipitator employed micro corona discharge at 1960 V and at less than 35 mu A to simultaneously charge, capture and lyse the airborne bacteria. This was enabled by the use of a 15 mu L. liquid anode. Using a custom exposure setup, the target bacterium Bacillus subtilis in the atomization solution was rendered airborne. After exposure, the liquid anode in the bio-precipitator was subsequently measured for DNA concentration and analyzed with the NanoGene assay. As the bacterial concentration increased from 0.0104 to 42.6 g-DCW/L the released DNA concentration in the liquid anode increased from 2.10 +/- 1.57 to 75.00 +/- 7.15 ng/mu L. More importantly, the NanoGene assay showed an increase in normalized fluorescence (gene quantification) from 18.03 +/- 1.18 to 49.71 +/- 1.82 as the bacterial concentrations increased from 0.0104 to 42.6 g-DCW/L. the electrical power consumption of the bioprecipitator was shown to be amenable for portable use. In addition, the detection limit of bio-precipitator and NanoGene assay combination in the context of environmentally relevant levels of airborne bacteria was also discussed. (C) 2016 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | LEGIONNAIRES-DISEASE | - |
dc.subject | ELECTROSTATIC PRECIPITATOR | - |
dc.subject | CELL-LYSIS | - |
dc.subject | PATHOGEN DETECTION | - |
dc.subject | BIOAEROSOLS | - |
dc.subject | DESIGN | - |
dc.subject | FUNGI | - |
dc.subject | AIR | - |
dc.subject | QUANTIFICATION | - |
dc.subject | COLLECTION | - |
dc.title | Detection of airborne bacteria with disposable bio-precipitator and NanoGene assay | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chua, Beelee | - |
dc.identifier.doi | 10.1016/j.bios.2016.04.051 | - |
dc.identifier.scopusid | 2-s2.0-84964545112 | - |
dc.identifier.wosid | 000376802300029 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.83, pp.205 - 212 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 83 | - |
dc.citation.startPage | 205 | - |
dc.citation.endPage | 212 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | PATHOGEN DETECTION | - |
dc.subject.keywordPlus | BIOAEROSOLS | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | FUNGI | - |
dc.subject.keywordPlus | AIR | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | COLLECTION | - |
dc.subject.keywordPlus | LEGIONNAIRES-DISEASE | - |
dc.subject.keywordPlus | ELECTROSTATIC PRECIPITATOR | - |
dc.subject.keywordPlus | CELL-LYSIS | - |
dc.subject.keywordAuthor | Bio-precipitator | - |
dc.subject.keywordAuthor | Airborne bacteria | - |
dc.subject.keywordAuthor | Micro corona discharge | - |
dc.subject.keywordAuthor | Bacillus subtilis | - |
dc.subject.keywordAuthor | NanoGene assay | - |
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