Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay
DC Field | Value | Language |
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dc.contributor.author | Park, Ok Hyun | - |
dc.contributor.author | Park, Joori | - |
dc.contributor.author | Yu, Mira | - |
dc.contributor.author | An, Hyoung-Tae | - |
dc.contributor.author | Ko, Jesang | - |
dc.contributor.author | Kim, Yoon Ki | - |
dc.date.accessioned | 2021-09-03T20:00:10Z | - |
dc.date.available | 2021-09-03T20:00:10Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-09-15 | - |
dc.identifier.issn | 0890-9369 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/87507 | - |
dc.description.abstract | Glucocorticoid (GC) receptor (GR) has been shown recently to bind a subset of mRNAs and elicit rapid mRNA degradation. However, the molecular details of GR-mediated mRNA decay (GMD) remain unclear. Here, we demonstrate that GMD triggers rapid degradation of target mRNAs in a translation-independent and exon junction complex independent manner, confirming that GMD is mechanistically distinct from nonsense-mediated mRNA decay (NMD). Efficient GMD requires PNRC2 (proline-rich nuclear receptor coregulatory protein 2) binding, helicase ability, and ATM-mediated phosphorylation of UPF1 (upstream frameshift 1). We also identify two GMD-specific factors: an RNA-binding protein, YBX1 (Y-box-binding protein 1), and an endoribonuclease, HRSP12 (heat-responsive protein 12). In particular, using HRSP12 variants, which are known to disrupt trimerization of HRSP12, we show that HRSP12 plays an essential role in the formation of a functionally active GMD complex. Moreover, we determine the hierarchical recruitment of GMD factors to target mRNAs. Finally, our genome-wide analysis shows that GMD targets a variety of transcripts, implicating roles in a wide range of cellular processes, including immune responses. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | - |
dc.subject | PROTEIN FAMILY YER057C/YIL051C/YJGF | - |
dc.subject | STRESS HORMONES | - |
dc.subject | DNA-DAMAGE | - |
dc.subject | ENHANCES TRANSLATION | - |
dc.subject | LIGAND-BINDING | - |
dc.subject | BREAST-CANCER | - |
dc.subject | UPF1 | - |
dc.subject | SURVEILLANCE | - |
dc.subject | COMPLEX | - |
dc.subject | CELLS | - |
dc.title | Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ko, Jesang | - |
dc.contributor.affiliatedAuthor | Kim, Yoon Ki | - |
dc.identifier.doi | 10.1101/gad.286484.116 | - |
dc.identifier.scopusid | 2-s2.0-84990942797 | - |
dc.identifier.wosid | 000385901400007 | - |
dc.identifier.bibliographicCitation | GENES & DEVELOPMENT, v.30, no.18, pp.2093 - 2105 | - |
dc.relation.isPartOf | GENES & DEVELOPMENT | - |
dc.citation.title | GENES & DEVELOPMENT | - |
dc.citation.volume | 30 | - |
dc.citation.number | 18 | - |
dc.citation.startPage | 2093 | - |
dc.citation.endPage | 2105 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Developmental Biology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Developmental Biology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | PROTEIN FAMILY YER057C/YIL051C/YJGF | - |
dc.subject.keywordPlus | STRESS HORMONES | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | ENHANCES TRANSLATION | - |
dc.subject.keywordPlus | LIGAND-BINDING | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | UPF1 | - |
dc.subject.keywordPlus | SURVEILLANCE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | glucocorticoid receptor-mediated mRNA decay | - |
dc.subject.keywordAuthor | YBX1 | - |
dc.subject.keywordAuthor | HRSP12 | - |
dc.subject.keywordAuthor | UPF1 | - |
dc.subject.keywordAuthor | PNRC2 | - |
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