Tumor necrosis factor- regulates interleukin-33 expression through extracellular signal-regulated kinase, p38, and nuclear factor-B pathways in airway epithelial cells
DC Field | Value | Language |
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dc.contributor.author | Park, Il-Ho | - |
dc.contributor.author | Park, Joo-Hoo | - |
dc.contributor.author | Shin, Jae-Min | - |
dc.contributor.author | Lee, Heung-Man | - |
dc.date.accessioned | 2021-09-03T20:22:03Z | - |
dc.date.available | 2021-09-03T20:22:03Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.issn | 2042-6976 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/87612 | - |
dc.description.abstract | BackgroundInterleukin (IL)-33 plays an important role in controlling immune responses in barrier tissues, and is a potent mediator of inflammatory diseases such as asthma, rheumatoid disease, and chronic rhinosinusitis. The aims of the present study were 2-fold: (1) to determine the stimulatory effect of tumor necrosis factor- (TNF-) on IL-33 production in nasal epithelial and A549 cells; and (2) to identify downstream pathways that activate IL-33 production. MethodsPrimary nasal epithelial cells (PNECs) from 5 normal patients were isolated and cultured. To identify which cytokines stimulate IL-33 production, we performed reverse-transcription polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence staining. Three mitogen-activated protein kinases (MAPKs) (p38, extracellular signal-regulated kinase [ERK], and c-Jun N-terminal kinase [JNK]) and nuclear factor B (NF-B) were evaluated as downstream signaling molecules by RT-PCR, ELISA, Western blot analysis, and luciferase reporter assay. ResultsThe IL-33 messenger RNA (mRNA) and protein levels were increased significantly by TNF- in PNECs and A549 cells. TNF- stimulated the expression of IL-33 in a dose- and time-dependent manner in A549 cells. PNECs and A549 cells were treated with TNF- in the presence of specific inhibitors of p38, ERK, JNK, and NF-B. In both cell types, inhibitors of ERK, p38, and NF-B reversed TNF--induced IL-33 production. In the luciferase reporter assay, NF-B activity was inhibited not only by an NF-B inhibitor, but also by ERK and p38 inhibitors. ConclusionTNF- stimulated IL-33 expression through ERK, p38, and NFB pathways in PNECs and A549 cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | CHRONIC RHINOSINUSITIS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | SINUS SURGERY | - |
dc.subject | IL-33 | - |
dc.subject | INFLAMMATION | - |
dc.subject | OUTCOMES | - |
dc.subject | MODEL | - |
dc.subject | MAPK | - |
dc.subject | ST2 | - |
dc.title | Tumor necrosis factor- regulates interleukin-33 expression through extracellular signal-regulated kinase, p38, and nuclear factor-B pathways in airway epithelial cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Il-Ho | - |
dc.contributor.affiliatedAuthor | Lee, Heung-Man | - |
dc.identifier.doi | 10.1002/alr.21761 | - |
dc.identifier.scopusid | 2-s2.0-84985963015 | - |
dc.identifier.wosid | 000383601000013 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY, v.6, no.9, pp.973 - 980 | - |
dc.relation.isPartOf | INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY | - |
dc.citation.title | INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY | - |
dc.citation.volume | 6 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 973 | - |
dc.citation.endPage | 980 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Otorhinolaryngology | - |
dc.relation.journalWebOfScienceCategory | Otorhinolaryngology | - |
dc.subject.keywordPlus | CHRONIC RHINOSINUSITIS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | SINUS SURGERY | - |
dc.subject.keywordPlus | IL-33 | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | OUTCOMES | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | MAPK | - |
dc.subject.keywordPlus | ST2 | - |
dc.subject.keywordAuthor | nose | - |
dc.subject.keywordAuthor | epithelium | - |
dc.subject.keywordAuthor | interleukin-33 | - |
dc.subject.keywordAuthor | tumor necrosis factor-alpha | - |
dc.subject.keywordAuthor | chronic rhinosinusitis | - |
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