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Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development

Authors
Kim, In JungKim, SangsooPark, JeahyunEom, IntaeKim, SunamKim, Jin-HongHa, Sung ChulKim, Yeon GilHwang, Kwang YeonNam, Ki Hyun
Issue Date
9월-2016
Publisher
WILEY-BLACKWELL
Keywords
biosensor; Dronpa; fluorescent protein; metal-binding site; quenching
Citation
FEBS LETTERS, v.590, no.17, pp.2982 - 2990
Indexed
SCIE
SCOPUS
Journal Title
FEBS LETTERS
Volume
590
Number
17
Start Page
2982
End Page
2990
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/87760
DOI
10.1002/1873-3468.12316
ISSN
0014-5793
Abstract
Many fluorescent proteins (FPs) show fluorescence quenching by specific metal ions, which can be applied towards metal biosensor development. In this study, we investigated the significant fluorescence quenching of Dronpa by Co2+ and Cu2+ ions. Crystal structures of Co2+-, Ni2+- and Cu2+ -bound Dronpa revealed previously unseen, unique, metal-binding sites for fluorescence quenching. These metal ions commonly interact with surface-exposed histidine residues (His194-His210 and His210-His212), and interact indirectly with chromophores. Structural analysis of the Co2+- and Cu2+- binding sites of Dronpa provides insight into FP-based metal biosensor engineering.
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