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Plasmonic-based colorimetric and spectroscopic discrimination of acetic and butyric acids produced by different types of Escherichia coli through the different assembly structures formation of gold nanoparticles

Authors
La, Ju A.Lim, SoraPark, Hyo JeongHeo, Min-JiSang, Byoung-InOh, Min-KyuCho, Eun Chul
Issue Date
24-8월-2016
Publisher
ELSEVIER SCIENCE BV
Keywords
Colorimetric discrimination and detection; Gold nanoparticles; Organic acids; Bacteria; Assembly of gold nanoparticles
Citation
ANALYTICA CHIMICA ACTA, v.933, pp.196 - 206
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICA CHIMICA ACTA
Volume
933
Start Page
196
End Page
206
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/87789
DOI
10.1016/j.aca.2016.05.035
ISSN
0003-2670
Abstract
We present a plasmonic-based strategy for the colourimetric and spectroscopic differentiation of various organic acids produced by bacteria. The strategy is based on our discovery that particular concentrations of DL-lactic, acetic, and butyric acids induce different assembly structures, colours, and optical spectra of gold nanoparticles. We selected wild-type (K-12 W3110) and genetically-engineered (JHL61) Escherichia coli (E. coli) that are known to primarily produce acetic and butyric acid, respectively. Different assembly structures and optical properties of gold nanoparticles were observed when different organic acids, obtained after the removal of acid-producing bacteria, were mixed with gold nanoparticles. Moreover, at moderate cell concentrations of K-12 W3110 E. coli, which produce sufficient amounts of acetic acid to induce the assembly of gold nanoparticles, a direct estimate of the number of bacteria was possible based on time-course colour change observations of gold nanoparticle aqueous suspensions. The plasmonic-based colourimetric and spectroscopic methods described here may enable onsite testing for the identification of organic acids produced by bacteria and the estimation of bacterial numbers, which have applications in health and environmental sciences. (C) 2016 Elsevier B.V. All rights reserved.
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