Kinetic studies on recombinant UDP-glucose: sterol 3-O-beta-glycosyltransferase from Micromonospora rhodorangea and its bioconversion potential
DC Field | Value | Language |
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dc.contributor.author | Nguyen Huu Hoang | - |
dc.contributor.author | Nguyen Lan Huong | - |
dc.contributor.author | Kim, Byul | - |
dc.contributor.author | Park, Je Won | - |
dc.date.accessioned | 2021-09-03T21:13:00Z | - |
dc.date.available | 2021-09-03T21:13:00Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-08-02 | - |
dc.identifier.issn | 2191-0855 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/87840 | - |
dc.description.abstract | Kinetics of a recombinant uridine diphosphate-glucose: sterol glycosyltransferase from Micromonospora rhodorangea ATCC 27932 (MrSGT) were studied using a number of sterols (including phytosterols) as glycosyl acceptors. The lowest K-m value and the highest catalytical efficiency (k(cat)/K-m) were found when beta-sitosterol was the glycosyl acceptor in the enzymatic reaction. In contrast to the enzyme's flexibility toward the glycosyl acceptor substrate, this recombinant enzyme was highly specific to uridine diphosphate (UDP)-glucose as the donor substrate. Besides, the UDP-glucose-dependent MrSGT was able to attach one glucose moiety specifically onto the C-3 hydroxyl group of other phytosterols such as fucosterol and gramisterol, yielding stereo-specific fucosterol-3-O-beta-D-glucoside and gramisterol-3-O-beta-D-glucoside, respectively. Based on kinetic data obtained from the enzyme's reactions using five different sterol substrates, the significance of the alkene (or ethylidene) side chains on the C-24 position in the sterol scaffolds was described and the possible relationship between the substrate structure and enzyme activity was discussed. This is the first report on the enzymatic bioconversion of the above two phytosteryl 3-O-beta-glucosides, as well as on the discovery of a stereospecific bacterial SGT which can attach a glucose moiety in beta-conformation at the C-3 hydroxyl group of diverse sterols, thus highlighting the catalytic potential of this promiscuous glycosyltransferase to expand the structural diversity of steryl glucosides. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.subject | HELICOBACTER-PYLORI | - |
dc.subject | WITHANIA-SOMNIFERA | - |
dc.subject | GLUCOSYLTRANSFERASE | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | ARABIDOPSIS | - |
dc.subject | FUCOSTEROL | - |
dc.title | Kinetic studies on recombinant UDP-glucose: sterol 3-O-beta-glycosyltransferase from Micromonospora rhodorangea and its bioconversion potential | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Je Won | - |
dc.identifier.doi | 10.1186/s13568-016-0224-x | - |
dc.identifier.scopusid | 2-s2.0-84982747990 | - |
dc.identifier.wosid | 000380979600002 | - |
dc.identifier.bibliographicCitation | AMB EXPRESS, v.6 | - |
dc.relation.isPartOf | AMB EXPRESS | - |
dc.citation.title | AMB EXPRESS | - |
dc.citation.volume | 6 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | HELICOBACTER-PYLORI | - |
dc.subject.keywordPlus | WITHANIA-SOMNIFERA | - |
dc.subject.keywordPlus | GLUCOSYLTRANSFERASE | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | FUCOSTEROL | - |
dc.subject.keywordAuthor | UDP-glucose sterol glycosyltransferase | - |
dc.subject.keywordAuthor | Kinetics | - |
dc.subject.keywordAuthor | Fucosterol-3-O-beta-D-glucoside | - |
dc.subject.keywordAuthor | Gramisterol-3-O-beta-D-glucoside | - |
dc.subject.keywordAuthor | Catalytic promiscuity | - |
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