Co-Culture of Tumor Spheroids and Fibroblasts in a Collagen Matrix-Incorporated Microfluidic Chip Mimics Reciprocal Activation in Solid Tumor Microenvironment
DC Field | Value | Language |
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dc.contributor.author | Jeong, Su-Yeong | - |
dc.contributor.author | Lee, Ji-Hyun | - |
dc.contributor.author | Shin, Yoojin | - |
dc.contributor.author | Chung, Seok | - |
dc.contributor.author | Kuh, Hyo-Jeong | - |
dc.date.accessioned | 2021-09-03T22:00:30Z | - |
dc.date.available | 2021-09-03T22:00:30Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-07-08 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/88076 | - |
dc.description.abstract | Multicellular 3D culture and interaction with stromal components are considered essential elements in establishing a 'more clinically relevant' tumor model. Matrix-embedded 3D cultures using a microfluidic chip platform can recapitulate the microscale interaction within tumor microenvironments. As a major component of tumor microenvironment, cancer-associated fibroblasts (CAFs) play a role in cancer progression and drug resistance. Here, we present a microfluidic chip-based tumor tissue culture model that integrates 3D tumor spheroids (TSs) with CAF in proximity within a hydrogel scaffold. HT-29 human colorectal carcinoma cells grew into 3D TSs and the growth was stimulated when co-cultured with fibroblasts as shown by 1.5-folds increase of % changes in diameter over 5 days. TS cultured for 6 days showed a reduced expression of Ki-67 along with increased expression of fibronectin when co-cultured with fibroblasts compared to mono-cultured TSs. Fibroblasts were activated under co-culture conditions, as demonstrated by increases in alpha-SMA expression and migratory activity. When exposed to paclitaxel, a survival advantage was observed in TSs co-cultured with activated fibroblasts. Overall, we demonstrated the reciprocal interaction between TSs and fibroblasts in our 7-channel microfluidic chip. The co-culture of 3D TS-CAF in a collagen matrix-incorporated microfluidic chip may be useful to study the tumor microenvironment and for evaluation of drug screening and evaluation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | CANCER-ASSOCIATED FIBROBLASTS | - |
dc.subject | STROMAL INTERACTIONS | - |
dc.subject | COLON-CANCER | - |
dc.subject | CELLS | - |
dc.subject | RESISTANCE | - |
dc.subject | CULTURE | - |
dc.subject | MODEL | - |
dc.subject | EXPRESSION | - |
dc.subject | SYSTEM | - |
dc.subject | POLYDIMETHYLSILOXANE | - |
dc.title | Co-Culture of Tumor Spheroids and Fibroblasts in a Collagen Matrix-Incorporated Microfluidic Chip Mimics Reciprocal Activation in Solid Tumor Microenvironment | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Seok | - |
dc.identifier.doi | 10.1371/journal.pone.0159013 | - |
dc.identifier.scopusid | 2-s2.0-84978796176 | - |
dc.identifier.wosid | 000380005400190 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.11, no.7 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 11 | - |
dc.citation.number | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | CANCER-ASSOCIATED FIBROBLASTS | - |
dc.subject.keywordPlus | STROMAL INTERACTIONS | - |
dc.subject.keywordPlus | COLON-CANCER | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | POLYDIMETHYLSILOXANE | - |
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