The effects of the antioxidant alpha-tocopherol succinate on cisplatin-induced ototoxicity in HEI-OC1 auditory cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Sung Kyun | - |
dc.contributor.author | Im, Gi Jung | - |
dc.contributor.author | An, Yun Suk | - |
dc.contributor.author | Lee, Se Hee | - |
dc.contributor.author | Jung, Hak Hyun | - |
dc.contributor.author | Park, Sang Yoo | - |
dc.date.accessioned | 2021-09-03T22:24:45Z | - |
dc.date.available | 2021-09-03T22:24:45Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 0165-5876 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/88204 | - |
dc.description.abstract | Conclusion: D-alpha-tocopherol succinate significantly reduced a cisplatin-induced hair cell loss in HEI-OC1 cell lines. These effects were mediated by its scavenging activity against reactive oxygen species (ROS) and inhibition of apoptosis. Objectives: Alpha-tocopherol is a class of methylated phenols, known as fat-soluble antioxidants, and is a different form of vitamin E, which reduces free radicals and acts as an antioxidant. We hypothesized that the antioxidative effect of alpha-tocopherol could protect against cisplastin-induced cytotoxicity, and thus evaluated its effects on cisplatin-induced ototoxicity in HEI-OC1 auditory cells. Methods: HEI-OC1 cells were pretreated with D-alpha-tocopherol succinate at a concentration of 10 mu M for 24 h, and then exposed to 15 mu M cisplatin for 48 h. The cellular viability was measured by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The intracellular ROS level was measured by using a fluorescent dye, 2',7'-dichlorofluorescein diacetate (DCFH-DA). Both Annexin V-FITC and propidium iodide (PI) staining were performed to analyze the pattern of apoptosis. The enzymatic activity of caspase-3 was assayed with caspase3/CPP32 fluorometric assay lot. Also, it was assessed by immunoblotting technique of poly-ADP-ribose polymerase (PARP). Results: Pretreatment with 10 mu M D-alpha-tocopherol succinate protected HEI-OC1 auditory cells against cisplatin-induced cytotoxicity. D-alpha-tocopherol succinate significantly reduced the cisplatin-induced increase in ROS. D-alpha-tocopherol succinate treatment induced a 15% reduction of ROS and 50% decrease in necrosis and late apoptosis as compared to cisplatin treatment. D-alpha-tocopherol succinate also decreased the activation of caspase-3 and reduced levels of cleaved poly-ADP-ribose polymerase (PARP). (C) 2016 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | INDUCED APOPTOSIS | - |
dc.subject | VITAMIN-E | - |
dc.subject | DEGENERATION | - |
dc.subject | CARBOPLATIN | - |
dc.subject | GROWTH | - |
dc.subject | SYSTEM | - |
dc.subject | LINE | - |
dc.title | The effects of the antioxidant alpha-tocopherol succinate on cisplatin-induced ototoxicity in HEI-OC1 auditory cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Im, Gi Jung | - |
dc.contributor.affiliatedAuthor | Jung, Hak Hyun | - |
dc.identifier.doi | 10.1016/j.ijporl.2016.04.008 | - |
dc.identifier.scopusid | 2-s2.0-84964689392 | - |
dc.identifier.wosid | 000378449000003 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY, v.86, pp.9 - 14 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY | - |
dc.citation.title | INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY | - |
dc.citation.volume | 86 | - |
dc.citation.startPage | 9 | - |
dc.citation.endPage | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Otorhinolaryngology | - |
dc.relation.journalResearchArea | Pediatrics | - |
dc.relation.journalWebOfScienceCategory | Otorhinolaryngology | - |
dc.relation.journalWebOfScienceCategory | Pediatrics | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | VITAMIN-E | - |
dc.subject.keywordPlus | DEGENERATION | - |
dc.subject.keywordPlus | CARBOPLATIN | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | LINE | - |
dc.subject.keywordAuthor | Alpha-tocopherol | - |
dc.subject.keywordAuthor | Cisplatin | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
dc.subject.keywordAuthor | Ototoxicity | - |
dc.subject.keywordAuthor | Antioxidants | - |
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