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Fibronectin on circulating extracellular vesicles as a liquid biopsy to detect breast cancer

Authors
Moon, Pyong-GonLee, Jeong-EunCho, Young-EunLee, Soo JungChae, Yee SooJung, Jin HyangKim, In-SanPark, Ho YongBaek, Moon-Chang
Issue Date
28-6월-2016
Publisher
IMPACT JOURNALS LLC
Keywords
breast cancer; diagnosis; extracellular vesicle; ELISA
Citation
ONCOTARGET, v.7, no.26, pp.40189 - 40199
Indexed
SCIE
SCOPUS
Journal Title
ONCOTARGET
Volume
7
Number
26
Start Page
40189
End Page
40199
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/88297
DOI
10.18632/oncotarget.9561
ISSN
1949-2553
Abstract
Extracellular vesicles (EVs) secreted from cancer cells have potential for generating cancer biomarker signatures. Fibronectin (FN) was selected as a biomarker candidate, due to the presence in surface on EVs secreted from human breast cancer cell lines. A subsequent study used two types of enzyme-linked immunosorbent assays (ELISA) to determine the presence of these proteins in plasma samples from disease-free individuals (n=70), patients with BC (n=240), BC patients after surgical resection (n=40), patients with benign breast tumor (n=55), and patients with non-cancerous diseases (thyroiditis, gastritis, hepatitis B, and rheumatoid arthritis; n=80). FN levels were significantly elevated (p<.0001) at all stages of BC, and returned to normal after tumor removal. The diagnostic accuracy for FN detection in extracellular vesicles (ELISA method 1) (area under the curve, 0.81; 95% CI, 0.76 to 0.86; sensitivity of 65.1% and specificity of 83.2%) were also better than those for FN detection in the plasma (ELISA method 2) (area under the curve, 0.77; 95% CI, 0.72 to 0.83; sensitivity of 69.2% and specificity of 73.3%) in BC. The diagnostic accuracy of plasma FN was similar in both the early-stage BC and all BC patients, as well as in the two sets. This liquid biopsy to detect FN on circulating EVs could be a promising method to detect early breast cancer.
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