Naringenin-induced migration of embrynoic trophectoderm cells is mediated via PI3K/AKT and ERK1/2 MAPK signaling cascades
- Authors
- Lim, Whasun; Song, Gwonhwa
- Issue Date
- 15-6월-2016
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- Naringenin; Trophectoderm cells; Migration; Peri-implantation
- Citation
- MOLECULAR AND CELLULAR ENDOCRINOLOGY, v.428, no.C, pp.28 - 37
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR AND CELLULAR ENDOCRINOLOGY
- Volume
- 428
- Number
- C
- Start Page
- 28
- End Page
- 37
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/88332
- DOI
- 10.1016/j.mce.2016.03.018
- ISSN
- 0303-7207
- Abstract
- For successful pregnancy, a well-coordinated network of growth factors, nutrients and hormones is required for fetal-maternal interactions. Naringenin, as a weak phytoestrogen, improves diabetes, inflammation, neuronal diseases, cardiovascular diseases and cancers. However, the role of naringenin in migration mechanism(s) of peri-implantation conceptuses is unknown. Therefore, in the present study, we determined the effects of naringenin on migration of porcine trophectoderm (pTr) cells, which is a known in vitro model for research on trophectoderm cell biology and placental-fetal developmental biology, in order to assess intracellular signal transduction pathways activated by naringenin. Migration of pTr cells increased in a dose-dependent manner in response to naringenin. Also, naringenin activated the phosphorylation of AKT and ERK1/2 proteins in a dose-dependent manner and those proteins were abundant mainly in the cytoplasm of naringenin-treated pTr cells. Within 30 min after treatment with 20 mu M naringenin, the abundance of phosphorylated EKR1/2, P70S6K, P9ORSK and S6K proteins increased, and then returned to basal levels by 120 min whereas the abundance of AKT increased gradually to 120 min post-treatment. However, the phosphorylation of AKT, P70S6K, P9ORSK and S6K was reduced in naringenin-induced pTr cells pre-treated with a PI3K inhibitor (LY294002). Also, a MEK1/2 inhibitor (U0126) significantly decreased naringenin-induced phosphorylation of ERK1/2, P70S6K and S6K proteins in pTr cells. Moreover, the naringenin-stimulated migration of pTr cells was suppressed by LY294002 and U0126. Collectively, results of the present study suggest that naringenin supports migration of pTr cells through PI3K/AKT and ERK1/2 MAPK signaling pathways crucial for orchestrating conceptus-uterine interactions. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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