Inhibition of c-Yes Induces Differentiation of HT-29 Human Colon Cancer Stem Cells through Midbody Elongation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Jessica | - |
dc.contributor.author | Choi, Sung Chul | - |
dc.contributor.author | Lee, Han-Na | - |
dc.contributor.author | Han, Gi-Yeon | - |
dc.contributor.author | Kim, Chan-Wha | - |
dc.date.accessioned | 2021-09-03T23:31:47Z | - |
dc.date.available | 2021-09-03T23:31:47Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-06 | - |
dc.identifier.issn | 1738-2696 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/88566 | - |
dc.description.abstract | Recent research suggests that a small group of cells, named cancer stem cells (CSCs), is responsible for initiating tumor formation, recurrence, and metastasis. c-Yes, a proto-oncogene that is a subfamily of Src family kinase, is often activated in human colon cancer; this implicates c-Yes in the onset and progression of the disease. The objective of this study was to investigate the correlation between c-Yes and CSCs. We performed a sphere formation assay and reverse transcription-polymerase chain reaction for studying the differentiation of HT-29 human colon CSCs. To demonstrate the specific role of c-Yes in CSCs, we performed live cell microscopy and a cell cycle assay. These study shows, for the first time, that c-Yes is enriched in CD133(+) CSCs, compared to their CD133(-) counterparts, and that c-Yes depletion in CD133(+) cells induces cell differentiation. Moreover, c-Yes depletion was found to elongate the midbody and increase the proliferation doubling time. This also suggested that the misregulation of microtubules during chromosomal separation causes aneuploidy. Our results suggest that c-Yes may play a crucial role in initiating, maintaining, and driving the tumorigenic property of colon cancer. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC | - |
dc.subject | TYROSINE KINASE-ACTIVITY | - |
dc.subject | SRC-FAMILY | - |
dc.subject | CYTOKINESIS | - |
dc.subject | ACTIVATION | - |
dc.subject | GROWTH | - |
dc.subject | MARKER | - |
dc.subject | CD133 | - |
dc.title | Inhibition of c-Yes Induces Differentiation of HT-29 Human Colon Cancer Stem Cells through Midbody Elongation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chan-Wha | - |
dc.identifier.doi | 10.1007/s13770-016-9053-x | - |
dc.identifier.scopusid | 2-s2.0-84973332890 | - |
dc.identifier.wosid | 000377468200007 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.13, no.3, pp.261 - 269 | - |
dc.relation.isPartOf | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.title | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.volume | 13 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 261 | - |
dc.citation.endPage | 269 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002112154 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.subject.keywordPlus | TYROSINE KINASE-ACTIVITY | - |
dc.subject.keywordPlus | SRC-FAMILY | - |
dc.subject.keywordPlus | CYTOKINESIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | MARKER | - |
dc.subject.keywordPlus | CD133 | - |
dc.subject.keywordAuthor | Cancer stem cell | - |
dc.subject.keywordAuthor | Src family kinase | - |
dc.subject.keywordAuthor | c-Yes | - |
dc.subject.keywordAuthor | Cell cycle | - |
dc.subject.keywordAuthor | Differentiation | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.