Enhanced thermostability of mesophilic endoglucanase Z with a high catalytic activity at active temperatures
DC Field | Value | Language |
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dc.contributor.author | Kim, Su Jung | - |
dc.contributor.author | Joo, Ji Eun | - |
dc.contributor.author | Jeon, Sang Duck | - |
dc.contributor.author | Hyeon, Jeong Eun | - |
dc.contributor.author | Kim, Seung Wook | - |
dc.contributor.author | Um, Young Soon | - |
dc.contributor.author | Han, Sung Ok | - |
dc.date.accessioned | 2021-09-03T23:59:56Z | - |
dc.date.available | 2021-09-03T23:59:56Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-05 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/88706 | - |
dc.description.abstract | This is the first study for therrmostable mutants of mesophilic endoglucanase EngZ from Clostridium cellulovorans using by site-directed mutagenesis. K94R, S365P and their double mutant K94R/S365P had a wide range of active temperatures (30-60 degrees C). In addition, the optimal temperature of K94R/S365P was increased by 7.5 degrees C. K94R/S365P retained 78.3% relative activity at 70 degrees C, while the wild type retained only 5.8%. Especially, K94R/S365P remained 45.1-fold higher activity than the wild type at 70 degrees C. In addition, K94R/S365P was 3.1-fold higher activity than the wild type at 42.5 degrees C, which is the optimal temperature of the wild type. K94R/S365P showed also stimulated in 2.5-fold lower concentration of CaCl2 and delayed aggregation temperature in the presence of CaCl2 compared to the wild type. In pH stability, K94R/S365P was not influenced, but the optimum pH was transferred from pH 7 to pH 6. In long-term hydrolysis, K94R/S365P reduced the newly released reducing sugar yields after 12 h reaction; however, the yields consistently increased until 72 h. Finally, the total reducing sugar of K94R/S365P was 5.0-fold higher than the wild type at 50 degrees C, pH6. EngZ (K94R/S365P) can support information to develop thermostability of GH9 endoglucanase with a high catalytic efficiency as the potential industrial bioprocess candidate. (C) 2016 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | CELL-SURFACE | - |
dc.subject | HYDROLYSIS | - |
dc.subject | ACID | - |
dc.subject | COMPLEXES | - |
dc.subject | PROTEINS | - |
dc.subject | DISPLAY | - |
dc.title | Enhanced thermostability of mesophilic endoglucanase Z with a high catalytic activity at active temperatures | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hyeon, Jeong Eun | - |
dc.contributor.affiliatedAuthor | Kim, Seung Wook | - |
dc.contributor.affiliatedAuthor | Han, Sung Ok | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2016.01.068 | - |
dc.identifier.scopusid | 2-s2.0-84956611496 | - |
dc.identifier.wosid | 000374196100033 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.86, pp.269 - 276 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 86 | - |
dc.citation.startPage | 269 | - |
dc.citation.endPage | 276 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | CELL-SURFACE | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | DISPLAY | - |
dc.subject.keywordAuthor | EngZ | - |
dc.subject.keywordAuthor | Thermostability | - |
dc.subject.keywordAuthor | Cellulolytic efficiency | - |
dc.subject.keywordAuthor | Family 9 endoglucanase | - |
dc.subject.keywordAuthor | Site-directed mutagenesis | - |
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