Characterization of Functional Domains in NME1L Regulation of NF-kappa B Signaling
DC Field | Value | Language |
---|---|---|
dc.contributor.author | You, Dong-Joo | - |
dc.contributor.author | Park, Cho Rong | - |
dc.contributor.author | Mander, Sunam | - |
dc.contributor.author | Ahn, Curie | - |
dc.contributor.author | Seong, Jae Young | - |
dc.contributor.author | Hwang, Jong-Ik | - |
dc.date.accessioned | 2021-09-04T00:08:49Z | - |
dc.date.available | 2021-09-04T00:08:49Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-05 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/88770 | - |
dc.description.abstract | NME1 is a well-known metastasis suppressor which has been reported to be downregulated in some highly aggressive cancer cells. Although most studies have focused on NME1, the NME1 gene also encodes the protein (NME1L) containing N-terminal 25 extra amino acids by alternative splicing. According to previous studies, NME1L has potent anti-metastatic activity, in comparison with NME1, by interacting with IKK beta and regulating its activity. In the present study, we tried to define the role of the N-terminal 25 amino acids of NME1L in NF-kappa B activation signaling. Unfortunately, the sequence itself did not interact with IKK beta, suggesting that it may be not enough to constitute the functional structure. Further construction of NME1L fragments and biochemical analysis revealed that N-terminal 84 residues constitute minimal structure for homodimerization, IKK beta interaction and regulation of NF-kappa B signaling. The inhibitory effect of the fragment on cancer cell migration and NF-kappa B-stimulated gene expression was equivalent to that of whole NME1L. The data suggest that the N-terminal 84 residues may be a core region for the anti-metastatic activity of NME1L. Based on this result, further structural analysis of the binding between NME1L and IKK beta may help in understanding the anti-metastatic activity of NME1L and provide direction to NME1L and IKK beta-related anti-cancer drug design. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.subject | NUCLEOSIDE DIPHOSPHATE KINASE | - |
dc.subject | TUMOR-METASTASIS SUPPRESSOR | - |
dc.subject | CANCER-CELLS | - |
dc.subject | BREAST-CANCER | - |
dc.subject | NM23-H1 | - |
dc.subject | PROTEIN | - |
dc.subject | EXPRESSION | - |
dc.subject | GENE | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | MECHANISM | - |
dc.title | Characterization of Functional Domains in NME1L Regulation of NF-kappa B Signaling | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seong, Jae Young | - |
dc.contributor.affiliatedAuthor | Hwang, Jong-Ik | - |
dc.identifier.doi | 10.14348/molcells.2016.2320 | - |
dc.identifier.scopusid | 2-s2.0-84983032436 | - |
dc.identifier.wosid | 000377104100006 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.39, no.5, pp.403 - 409 | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 39 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 403 | - |
dc.citation.endPage | 409 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002106899 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | NUCLEOSIDE DIPHOSPHATE KINASE | - |
dc.subject.keywordPlus | TUMOR-METASTASIS SUPPRESSOR | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | NM23-H1 | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordAuthor | anti-metastasis | - |
dc.subject.keywordAuthor | dimerization | - |
dc.subject.keywordAuthor | IKK beta | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | NME1L | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.