Recombinant production of a shell matrix protein in Escherichia coli and its application to the biomimetic synthesis of spherulitic calcite crystals
DC Field | Value | Language |
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dc.contributor.author | Song, Wooho | - |
dc.contributor.author | Bahn, So Yeong | - |
dc.contributor.author | Cha, Hyung Joon | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.contributor.author | Choi, Yoo Seong | - |
dc.date.accessioned | 2021-09-04T00:18:23Z | - |
dc.date.available | 2021-09-04T00:18:23Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-05 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/88841 | - |
dc.description.abstract | Objectives To overcome the limited production capability of shell matrix proteins and efficiently conduct in vitro CaCO3 biomineralization studies, a putative recombinant shell matrix protein was prepared and characterized. Results A glycine-rich protein (GRP_BA) was found in Pinctada fucata as a putative shell matrix protein (NCBI reference sequence; BAA20465). It was genetically redesigned for the production in Escherichia coli. The recombinant protein was obtained in a 400 ml shake-flask culture at approx. 30 mg l(-1) with a purity of > 95 %. It efficiently formed a complex with Ca2+. Ca2+-induced agglomeration was like other calcification-related proteins. Spherulitic calcite micro-particles, 20-30 A mu m diam. with rosette- and sphere-like structures were synthesized in the presence of the recombinant shell protein, which could be formed by stacking and/or aggregation of calcite nanograins and the bound protein. Conclusions Recombinant production of a shell matrix protein could overcome potential difficulties associated with the limited amount of protein available for biomineralization studies and provide opportunities to fabricate biominerals in practical aspects. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | CRYSTALLIZATION | - |
dc.subject | ORIGIN | - |
dc.subject | MODEL | - |
dc.title | Recombinant production of a shell matrix protein in Escherichia coli and its application to the biomimetic synthesis of spherulitic calcite crystals | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1007/s10529-016-2039-x | - |
dc.identifier.scopusid | 2-s2.0-84954322260 | - |
dc.identifier.wosid | 000373857600010 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY LETTERS, v.38, no.5, pp.809 - 816 | - |
dc.relation.isPartOf | BIOTECHNOLOGY LETTERS | - |
dc.citation.title | BIOTECHNOLOGY LETTERS | - |
dc.citation.volume | 38 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 809 | - |
dc.citation.endPage | 816 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | CRYSTALLIZATION | - |
dc.subject.keywordPlus | ORIGIN | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordAuthor | Biomineralization | - |
dc.subject.keywordAuthor | Calcium carbonate | - |
dc.subject.keywordAuthor | Pearl oyster | - |
dc.subject.keywordAuthor | Recombinant protein | - |
dc.subject.keywordAuthor | Spherulitic calcite crystal | - |
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