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BODIPY/Nile-Red-Based Efficient FRET Pair: Selective Assay of Endoplasmic Reticulum Membrane Fluidity
- Authors
- Yang, Zhigang; Wi, Youngjin; Yoon, Young-Min; Verwilst, Peter; Jang, Joo Hee; Kim, Tae Woo; Kang, Chulhun; Kim, Jong Seung
- Issue Date
- 18-2월-2016
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- endoplasmic reticulum; fluorescence; FRET; membrane fluidity; metabolic syndrome
- Citation
- CHEMISTRY-AN ASIAN JOURNAL, v.11, no.4, pp.527 - 531
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHEMISTRY-AN ASIAN JOURNAL
- Volume
- 11
- Number
- 4
- Start Page
- 527
- End Page
- 531
- ISSN
- 1861-4728
- Abstract
- We synthesized a boron-dipyrromethene (BODIPY)/Nile Red hybrid probe capable of selectively recognizing fluidity changes in the endoplasmic reticulum (ER) membrane due to its preferential localization to the ER and strong energy transfer from BODIPY to the Nile Red moiety, emitting only in nonaqueous environments. ER membrane fluidity in HepG2 cells was markedly reduced by a cell model of metabolic syndrome.
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